Kirkland J L, Mukku V R, Hardy M, Stancel G M
Endocrinology. 1984 Mar;114(3):969-73. doi: 10.1210/endo-114-3-969.
Administration of a single injection of estradiol (E2) causes a maximum increase in DNA synthesis in the uterine luminal epithelium approximately 24 h later. We previously reported that animals receiving a second injection of E2 15-18 h after the first show an apparent decrease in DNA synthesis measured in this cell type at 24 h. This apparent decrease in DNA synthesis is due to a shift in the time course of DNA synthesis rather than an absolute decrease in this parameter. In this report we demonstrate that this inhibitory effect of a second injection of E2 is also observed after intraluminal instillation of the hormone. This effect is dose dependent and appears to result from a shift in the time course of luminal epithelial DNA synthesis. The intraluminal instillation of E2 produces the same pattern of nuclear receptor localization as sc administration of the hormone. These results suggest that this inhibitory effect we previously described results primarily from a direct action of E2 on the luminal epithelium.
单次注射雌二醇（E2）后，大约24小时后子宫腔上皮中的DNA合成会出现最大程度的增加。我们之前报道过，在第一次注射E2后15 - 18小时接受第二次注射的动物，在24小时时这种细胞类型中测得的DNA合成明显减少。DNA合成的这种明显减少是由于DNA合成时间进程的改变，而非该参数的绝对减少。在本报告中，我们证明在激素腔内滴注后也观察到第二次注射E2的这种抑制作用。这种作用是剂量依赖性的，似乎是由腔上皮DNA合成时间进程的改变导致的。E2的腔内滴注产生的核受体定位模式与皮下注射该激素相同。这些结果表明，我们之前描述的这种抑制作用主要是由E2对腔上皮的直接作用引起的。
