Mukku V R, Kirkland J L, Hardy M, Stancel G M
Endocrinology. 1982 Aug;111(2):480-7. doi: 10.1210/endo-111-2-480.
Administration of a single injection of estradiol (E2) causes a maximum increase in DNA synthesis of all major uterine cell types approximately 24 h later. Animals given a second injection of E2 6-12 h after the first show an apparent increase in DNA synthesis in the luminal epithelium. Animals receiving a second injection of E2 15-18 h after the first show an apparent decrease in DNA synthesis at 24 h, which is most prominent in the luminal epithelium. This apparent decrease in DNA synthesis is most apparent if the second injection of E2 is given 18 h after the first, and is due to a shift of 10-12 h in the time course of DNA synthesis rather than to an absolute decrease in this parameter. This shift in the time course of uterine DNA synthesis is a dose-dependent phenomenon and displays a dose-response curve similar to that for the stimulation of DNA synthesis by a single injection of E2. A third injection of E2, 28 h after the initial hormone treatment, again causes a shift of 10-12 h in the time course of DNA synthesis relative to that in animals receiving two injections of hormone. These results suggest that nuclear levels of estrogen receptor, which initially increase after hormone administration, must decrease before the onset of uterine DNA synthesis.
单次注射雌二醇（E2）后，大约24小时后所有主要子宫细胞类型的DNA合成会出现最大程度的增加。在首次注射后6 - 12小时接受第二次E2注射的动物，其腔上皮中的DNA合成明显增加。在首次注射后15 - 18小时接受第二次E2注射的动物，在24小时时DNA合成明显减少，这在腔上皮中最为显著。如果在首次注射后18小时给予第二次E2注射，这种DNA合成的明显减少最为明显，这是由于DNA合成时间进程发生了10 - 12小时的偏移，而不是该参数的绝对减少。子宫DNA合成时间进程的这种偏移是一种剂量依赖性现象，并且显示出与单次注射E2刺激DNA合成相似的剂量反应曲线。在初始激素处理后28小时进行第三次E2注射，相对于接受两次激素注射的动物，DNA合成时间进程再次发生10 - 12小时的偏移。这些结果表明，激素给药后最初增加的雌激素受体核水平，在子宫DNA合成开始之前必须降低。
