Pesanti E L
Infect Immun. 1978 May;20(2):503-11. doi: 10.1128/iai.20.2.503-511.1978.
The effects of suramin on phagolysosome formation and antimicrobial activity of mouse peritoneal macrophages cultivated in vitro have been studied. Prolonged in vitro pretreatment of macrophages with high concentrations of suramin caused macrophages to form large fragile phagolysosomes in which the concentrations of the various lysosomal enzymes were inferred to be diminished. In addition, suramin-treated macrophages demonstrated enhanced exocytosis of acid phosphatase during phagocytosis of polyvinyl toluene spherules. However, suramin was found not to inhibit formation of phagolysosomes in macrophages that had ingested Listeria monocytogenes when those cells were examined by the electron microscope. Suramin pretreatment did not alter the ingestion or intracellular killing of Staphylococcus aureus or of a strain of L. monocytogenes that was essentially avirulent for mice, but did protect macrophages from destruction by virulent L. monocytogenes ingested in vitro, an effect that appeared to have been mediated through enhancement of the bacteriostatic potential of the macrophages. However, at a single dosage level, the drug did not alter the mortality of mice challenged with virulent L. monocytogenes.
研究了苏拉明对体外培养的小鼠腹腔巨噬细胞吞噬溶酶体形成及抗菌活性的影响。用高浓度苏拉明对巨噬细胞进行长时间体外预处理，会导致巨噬细胞形成大而脆弱的吞噬溶酶体，据推测其中各种溶酶体酶的浓度会降低。此外，经苏拉明处理的巨噬细胞在吞噬聚乙烯甲苯小球过程中，酸性磷酸酶的胞吐作用增强。然而，当通过电子显微镜检查时，发现苏拉明并不抑制已吞噬单核细胞增生李斯特菌的巨噬细胞中吞噬溶酶体的形成。苏拉明预处理并未改变巨噬细胞对金黄色葡萄球菌或对小鼠基本无毒力的单核细胞增生李斯特菌菌株的摄取或细胞内杀伤作用，但确实能保护巨噬细胞免受体外摄取的有毒力单核细胞增生李斯特菌的破坏，这种作用似乎是通过增强巨噬细胞的抑菌潜能介导的。然而，在单一剂量水平下，该药物并未改变感染有毒力单核细胞增生李斯特菌的小鼠的死亡率。
