Kotula A W, Murrell K D, Acosta-Stein L, Lamb L
J Anim Sci. 1984 Jan;58(1):94-8. doi: 10.2527/jas1984.58194x.
Thirty-two Hampshire-Yorkshire pigs (6 to 8 wk old) were inoculated with the Beltsville strain of Trichinella spiralis at a level of about 880 larvae/kg of body weight (about 15 kg). At about 100 kg, the pigs were slaughtered and 10-g samples of muscle and other tissues were digested in pepsin-HC1 and examined microscopically for T. spiralis larvae. The mean number of larvae recovered/gram was: tongue, 452; diaphragm, 391; obliquus abdominis internus, 130; serratus ventralis, 116; psoas major, 105; triceps brachii, 100; biceps femoris, 83; semitendinosus, 74; intercostal, 60; semimembranosus, 58 and longissimus dorsi, 37. The liver and spleen samples contained none. Larvae were found in one sample each of the blood, brain and kidney, in two samples of the heart, and in four samples of lymph tissue. Each of these samples was from a different pig except the positive samples of brain and heart, which were from the same pig. The larvae found in the blood, brain, kidney, heart and lymph were first stage larvae and, therefore, do not indicate migration of newborn larvae from the gut. The presence of these larvae in non-striated muscle tissue may have been due to contamination of the organs from infected skeletal muscle. These data confirmed previous reports of the distribution of the T. spiralis larvae among individual muscles of the carcass. Further, the data suggest that cross-contamination of organ tissue is possible during evisceration and, therefore, organ meat from infected swine cannot be assured to be free of T. spiralis larvae.
选用32头汉普夏-约克夏猪（6至8周龄），以约880条幼虫/千克体重（猪体重约15千克）的剂量接种旋毛虫贝兹维尔株。猪体重约达100千克时屠宰，取10克肌肉和其他组织样本，用胃蛋白酶-盐酸消化后镜检旋毛虫幼虫。每克组织回收幼虫的平均数为：舌部452条；膈肌391条；腹内斜肌130条；腹侧锯肌116条；腰大肌105条；肱三头肌100条；股二头肌83条；半腱肌74条；肋间肌60条；半膜肌58条；背最长肌37条。肝脏和脾脏样本未发现幼虫。血液、脑和肾各有1个样本发现幼虫，心脏有2个样本发现幼虫，淋巴组织有4个样本发现幼虫。除脑和心脏的阳性样本来自同一头猪外，其他这些样本均来自不同的猪。在血液、脑、肾、心脏和淋巴中发现的幼虫为第一期幼虫，因此并不表明新生幼虫从肠道迁移。在非横纹肌组织中发现这些幼虫可能是由于受感染的骨骼肌污染了这些器官。这些数据证实了先前关于旋毛虫幼虫在胴体各肌肉中分布情况的报道。此外，这些数据表明，在摘除内脏过程中器官组织可能会发生交叉污染，因此，感染猪的脏器肉不能保证无旋毛虫幼虫。
