Kapel C M, Webster P, Lind P, Pozio E, Henriksen S A, Murrell K D, Nansen P
Danish Centre for Experimental Parasitology, The Royal Veterinary and Agricultural University, Frederiksberg C.
Parasitol Res. 1998;84(4):264-71. doi: 10.1007/s004360050393.
The infectivity of Trichinella spiralis, T. nativa, and T. britovi was experimentally compared in pigs. Blood sampling was performed weekly, and muscle juices were obtained at slaughter 10 weeks after inoculation. Muscle larvae were found in all of four pigs inoculated with T. spiralis [mean 190 larvae per gram (lpg)] and in three of four pigs inoculated with T. britovi (mean 7 lpg). No larvae were found in pigs inoculated with T. nativa. For T. spiralis and T. britovi, the neck muscle (m. splenius) appears to be a predilection site in addition to the tongue, the diaphragm, and the jaw. High antibody responses were found in all experimental groups, independent of the antigen used, and even in pigs in which no muscle larvae were recovered. The strong and consistent antibody response found with meat juice indicates the usefulness of this material where a blood sample is not obtainable, e.g. meat samples from wild animals. Immunoblotting (Western blots) on slaughter sera revealed no species specificity when comparing homologous versus heterologous staining.
在猪身上对旋毛虫、本地旋毛虫和布氏旋毛虫的感染性进行了实验比较。每周进行采血，在接种后10周屠宰时获取肌肉汁液。在接种旋毛虫的所有4头猪中均发现了肌肉幼虫（平均每克190条幼虫），在接种布氏旋毛虫的4头猪中有3头发现了肌肉幼虫（平均每克7条幼虫）。接种本地旋毛虫的猪中未发现幼虫。对于旋毛虫和布氏旋毛虫，除了舌头、膈肌和颌部外，颈部肌肉（夹肌）似乎也是一个偏好寄生部位。在所有实验组中均发现了高抗体反应，与所用抗原无关，甚至在未检出肌肉幼虫的猪中也是如此。用肉汁检测到的强烈且一致的抗体反应表明，在无法获取血样的情况下，例如野生动物的肉样，这种材料是有用的。对屠宰血清进行免疫印迹（蛋白质印迹）分析发现，在比较同源染色与异源染色时没有种属特异性。
