脱氧核糖核酸复制缺陷的温度敏感型大肠杆菌突变体的分离与鉴定。
Isolation and characterization of thermosensitive Escherichia coli mutants defective in deoxyribonucleic acid replication.
作者信息
Wechsler J A, Nüsslein V, Otto B, Klein A, Bonhoeffer F, Herrmann R, Gloger L, Schaller H
出版信息
J Bacteriol. 1973 Mar;113(3):1381-8. doi: 10.1128/jb.113.3.1381-1388.1973.
Abstract
Thermosensitive deoxyribonucleic acid replication-defective mutants have been isolated by using an autoradiographic selection method. The mutants have been analyzed genetically and biochemically. Some of the mutants show thermosensitivity of in vitro deoxyribonucleic acid replication. These can be classified into three groups according to their behavior in in vitro complementation assays. This classification is congruent with that obtained by genetic mapping by using cotransduction frequencies with selected markers in P1 transduction analysis.
摘要
利用放射自显影选择法已分离出热敏性脱氧核糖核酸复制缺陷型突变体。已对这些突变体进行了遗传和生化分析。一些突变体表现出体外脱氧核糖核酸复制的热敏性。根据它们在体外互补试验中的行为，这些突变体可分为三组。这种分类与通过在P1转导分析中使用与选定标记的共转导频率进行遗传作图所获得的分类一致。
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