Hambrecht G S, Hilton J G
Life Sci. 1984 Mar 5;34(10):971-6. doi: 10.1016/0024-3205(84)90301-1.
We report here a modification of the superfused hamster cheek model for use in vascular permeability studies. Radio-iodine labeled serum albumin (I-125 RISA) is injected prior to the superfusion period. Plasma content is calculated on a microliter/100 mg wet weight basis and compared to the contralateral (non-superfused) cheek pouch. Water content is calculated on a percentage basis and compared in the same manner. Results demonstrate that superfusion causes an increase in permeability of protein and water. Plasma content is reduced by catalase, indomethacin or FPL 55712 pretreatment, suggesting that free-radicals, prostaglandins and leukotrienes are released during superfusion. Water content increase is refractory to pretreatment. The advantages of this system and its application are discussed.
我们在此报告一种经改良的用于血管通透性研究的灌注仓鼠颊囊模型。在灌注期之前注射放射性碘标记的血清白蛋白（I-125 RISA）。血浆含量以微升/100毫克湿重为基础进行计算，并与对侧（未灌注）颊囊进行比较。含水量以百分比为基础进行计算，并以相同方式进行比较。结果表明，灌注会导致蛋白质和水的通透性增加。过氧化氢酶、吲哚美辛或FPL 55712预处理可降低血浆含量，这表明在灌注过程中会释放自由基、前列腺素和白三烯。含水量的增加对预处理具有抗性。本文讨论了该系统的优点及其应用。
