曼氏血吸虫和肝片吸虫这两种吸虫中蜕皮甾体的分析。

Analysis of ecdysteroids in the trematodes, Schistosoma mansoni and Fasciola hepatica.

作者信息

Foster J M, Mercer J G, Rees H H

机构信息

Department of Biochemistry, University of Liverpool, U.K.

出版信息

Trop Med Parasitol. 1992 Dec;43(4):239-44.

PMID:1293728

Abstract

Adult Schistosoma mansoni from experimentally infected mice and Fasciola hepatica recovered from ovine livers post mortem were analyzed for free and conjugated ecdysteroids by radioimmunoassay, high-performance liquid chromatography monitoring fractions by radioimmunoassay, and by capillary gas chromatography/mass spectrometry (selected ion monitoring). Both species contained ecdysone and 20-hydroxyecdysone as free ecdysteroids and as polar conjugates. F. hepatica also contained a polar conjugate of 2-deoxyecdysone. Evidence of apolar ecdysteroid conjugates was only obtained for F. hepatica. The free ecdysteroid-containing fraction of S. mansoni contained small amounts of unidentified immunoreactive material.

摘要

通过放射免疫测定、利用放射免疫测定监测馏分的高效液相色谱法以及毛细管气相色谱/质谱法（选择离子监测），对来自实验感染小鼠的成年曼氏血吸虫和死后从绵羊肝脏中回收的肝片吸虫的游离和结合蜕皮甾体进行了分析。这两种物种都含有蜕皮激素和20-羟基蜕皮激素，作为游离蜕皮甾体和极性共轭物。肝片吸虫还含有2-脱氧蜕皮激素的极性共轭物。仅在肝片吸虫中获得了非极性蜕皮甾体共轭物的证据。曼氏血吸虫含游离蜕皮甾体的馏分含有少量未鉴定的免疫反应性物质。