Globomycin, a new peptide antibiotic with spheroplast-forming activity. I. Taxonomy of producing organisms and fermentation.

A new peptide antibiotic, globomycin, was found to be produced by four different strains of the actinomycetes. They were identified as Streptomyces halstedii No. 13912, Streptoverticillium cinnamoneum No. 15037, Streptomyces neohygroscopicus subsp. globomyceticus No. 15631 and Streptomyces hagronensis No. 17834, respectively. Fermentation of globomycin was conducted by conventional submerged culture for antibiotic production, in which 10 microgram/ml of globomycin was produced by cultivation of S. halstedii No. 13912 for 96 hours at 27 degrees C. Globomycin was named after its activity to form global-shape spheroplasts when Escherichia coli was incubated in the presence of this antibiotic.