Fischer E J, Hilwig I, Leschinski E
Endocrinology. 1977 Jun;100(6):1659-62. doi: 10.1210/endo-100-6-1659.
beta-Cells may be demonstrated in pancreas sections as well as in monolayer cultures. Until now, however, it has been impossible to differentiate quickly among individual cell types in freshly dissociated cell suspensions prepared for pancreatic monolayer cultures. Rapid identification of endocrine cells within the total cell population can be achieved by means of vital staining with neutral red. Moreover, the direct observation of unfixed and unstained cell suspensions under dark-field illumination allows an immediate identification of granulated beta-cells.
β细胞在胰腺切片以及单层培养物中均可被显示出来。然而，到目前为止，要在为胰腺单层培养准备的新鲜解离的细胞悬液中的单个细胞类型之间进行快速区分是不可能的。通过用中性红进行活体染色，可以在整个细胞群体中快速识别内分泌细胞。此外，在暗视野照明下对未固定和未染色的细胞悬液进行直接观察，可以立即识别出有颗粒的β细胞。
