• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Reverse phase high pressure liquid chromatography for the separation of peptide hormone diastereoisomers.

作者信息

Larsen B, Viswanatha V, Chang S Y, Hruby V J

出版信息

J Chromatogr Sci. 1978 May;16(5):207-10. doi: 10.1093/chromsci/16.5.207.

DOI:10.1093/chromsci/16.5.207
PMID:670372
Abstract

Diastereoisomers of specifically labeled oxytocin derivatives were resolved using reverse phase high pressure liquid chromatography. The peptides [1-hemi-DL-[alpha-2H]cystine]oxytocin, [6-hemi-DL-[alpha-2H]cystine]oxytocin, [2-DL-[alpha-2H]tyrosine]oxytocin and[8-DL-[2-13C]leucine]oxytocin were readily separated using the conditions described. The diastereoisomers of the oxytocin analog [3-DL-[2-13C]leucine]oxytocin also demonstrated baseline resolution under the same conditions. The procedure offers the investigator a rapid method for screening synthetic oxytocin peptides for undesirable diastereoisomeric by-products.

摘要

相似文献

1
Reverse phase high pressure liquid chromatography for the separation of peptide hormone diastereoisomers.
J Chromatogr Sci. 1978 May;16(5):207-10. doi: 10.1093/chromsci/16.5.207.
2
The separation of peptide hormone diastereoisomers by reverse phase high pressure liquid chromatography. Factors affecting separation of oxytocin and its diastereoisomers--structural implications.
Int J Pept Protein Res. 1979 Jan;13(1):12-21. doi: 10.1111/j.1399-3011.1979.tb01844.x.
3
Investigation of the interactions of oxytocin with neurophysins at low pH using carbon-13 nuclear magnetic resonance and carbon-13-labeled hormones.
Biochemistry. 1979 Aug 7;18(16):3552-7. doi: 10.1021/bi00583a018.
4
Simultaneous synthesis of 1-hemi-D-cystine-oxytocin and oxytocin and separation of the diastereoisomers by partition chromatography on sephadex and by countercurrent distribution.1-半胱氨酸-催产素和催产素的同时合成以及通过葡聚糖凝胶上的分配色谱法和逆流分配法分离非对映异构体。
J Am Chem Soc. 1966 Mar 20;88(6):1310-3. doi: 10.1021/ja00958a040.
5
[High performance liquid chromatography of peptide bioregulators, their fragments and derivatives. IV. Chromatographic behavior and purification of oxytocin].[肽类生物调节剂及其片段和衍生物的高效液相色谱法。IV. 催产素的色谱行为及纯化]
Bioorg Khim. 1989 Sep;15(9):1157-60.
6
Separation of 17 DL-amino acids and chiral sequential analysis of peptides by reversed-phase liquid chromatography after labeling with R(-)-4- (3-isothiocyanatopyrrolidin-1-yl)-7-(N, N-dimethylaminosulfonyl)-2,1,3-benzoxadiazole.用R(-)-4-(3-异硫氰酸酯基吡咯烷-1-基)-7-(N,N-二甲基氨基磺酰基)-2,1,3-苯并恶二唑标记后，通过反相液相色谱法分离17种DL-氨基酸并对肽进行手性序列分析。
Anal Biochem. 1999 Dec 1;276(1):48-58. doi: 10.1006/abio.1999.4307.
7
In vivo biosynthesis of L-[35S]Cys-arginine vasopressin, -oxytocin, and -somatostatin: rapid estimation using reversed phase high pressure liquid chromatography.L-[35S]半胱氨酸-精氨酸加压素、催产素和生长抑素的体内生物合成：使用反相高效液相色谱法进行快速测定。
Endocrinology. 1981 Oct;109(4):1097-106. doi: 10.1210/endo-109-4-1097.
8
Comparison of the diastereoisomeric excess of uridine, inosine and adenosine cyanohydrins determined by HPLC-DAD and H NMR.通过高效液相色谱-二极管阵列检测法（HPLC-DAD）和核磁共振氢谱（¹H NMR）测定尿苷、肌苷和腺苷氰醇非对映体过量的比较。
Nucleosides Nucleotides Nucleic Acids. 2017 Oct 3;36(10):652-665. doi: 10.1080/15257770.2017.1375516. Epub 2017 Nov 29.
9
Separation and characterization of diastereoisomeric oligonucleotide.非对映异构寡核苷酸的分离与表征
Nucleic Acids Symp Ser. 1992(27):137-8.
10
Separation and characterization of diastereoisomers of antisense oligodeoxyribonucleoside phosphorothioates.
Anal Biochem. 1994 Dec;223(2):285-90. doi: 10.1006/abio.1994.1586.

引用本文的文献

1
Analysis of Bacteriophage Behavior of a Human RNA Virus, SARS-CoV-2, through the Integrated Approach of Immunofluorescence Microscopy, Proteomics and D-Amino Acid Quantification.通过免疫荧光显微镜、蛋白质组学和 D-氨基酸定量分析综合方法分析人类 RNA 病毒 SARS-CoV-2 的噬菌体行为。
Int J Mol Sci. 2023 Feb 15;24(4):3929. doi: 10.3390/ijms24043929.
2
Boosting basic-peptide separation through dynamic electrostatic-repulsion reversed-phase (d-ERRP) liquid chromatography.通过动态静电排斥反相（d-ERRP）液相色谱法增强碱性肽的分离
RSC Adv. 2020 Mar 27;10(21):12604-12610. doi: 10.1039/d0ra01296c. eCollection 2020 Mar 24.