Yasuhara M, Fujita S, Arisue K, Kohda K, Hayashi C
Clin Biochem. 1984 Feb;17(1):31-3. doi: 10.1016/s0009-9120(84)90420-x.
A new enzymatic method is described for the determination of creatine in serum and urine with Abbott ABA-200. The measurement is accomplished by transforming creatine to formic acid in a reaction catalyzed by creatinase (creatine amidinohydrolase), sarcosine oxidase and formaldehyde dehydrogenase (see Figure 1). The assay takes less than 20 minutes. The standard response is linear for creatine concentrations up to 10 mg/dL (serum) and 80 mg/dL (urine). The coefficients of variation at 0.69 mg/dL (serum) and 4.93 mg/dL (urine) for within-day determination were less than 4% and, for between-day determination, were less than 5%. Results obtained by this procedure on one hundred serum and urine samples conformed well with a manual enzymatic method and the Folin method. The method is useful for the automated measurement of creatine in serum and urine.
本文描述了一种使用雅培ABA - 200测定血清和尿液中肌酸的新酶法。该测量通过在肌酸酶（肌酸脒水解酶）、肌氨酸氧化酶和甲醛脱氢酶催化的反应中将肌酸转化为甲酸来完成（见图1）。该测定耗时不到20分钟。对于血清中高达10 mg/dL和尿液中高达80 mg/dL的肌酸浓度，标准响应呈线性。日内测定在0.69 mg/dL（血清）和4.93 mg/dL（尿液）时的变异系数小于4%，日间测定时小于5%。用该方法对100份血清和尿液样本进行检测的结果与手工酶法和福林法的结果非常吻合。该方法可用于血清和尿液中肌酸的自动化测量。
