Yasuhara M, Fujita S, Furukawa I, Arisue K, Kohda K, Hayashi C
Clin Chem. 1981 Nov;27(11):1888-91.
A new enzymic method is described for the determination of creatine in urine by continuous-flow analysis. The measurement is accomplished by transforming creatine to formaldehyde in reactions catalyzed by creatinase (creatine amidinohydrolase) and sarcosine dehydrogenase. The formaldehyde is reacted with 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole to form a purple product, which is measured colorimetrically. The method gives a linear standard curve for creatine concentrations up to 100 mg/L. Precision and analytical recovery are excellent, and results correlate well with those by the more-difficult Folin method, which currently is most often used for this analysis.
本文描述了一种通过连续流动分析测定尿液中肌酸的新酶法。该测量通过在肌酸酶（肌酸脒水解酶）和肌氨酸脱氢酶催化的反应中将肌酸转化为甲醛来完成。甲醛与4-氨基-3-肼基-5-巯基-1,2,4-三唑反应形成紫色产物，用比色法测定。该方法在肌酸浓度高达100mg/L时给出线性标准曲线。精密度和分析回收率极佳，结果与目前最常用于此分析的更复杂的福林法的结果相关性良好。
