Friedlander M J, Stanford L R
Exp Brain Res. 1984;53(2):451-61. doi: 10.1007/BF00238175.
Six cats were reared with monocular eyelid suture from 1 week of age. Two control animals were reared in the same colony. The sutured eye of the monocularly deprived (MD) cats was opened at 8-9 months of age. The effect of monocular deprivation on the distribution of functional classes of neurons in the dorsal lateral geniculate nucleus (LGNd) was evaluated by extracellularly recording the activity of single neurons in the A-laminae of the LGNd ipsilateral to the deprived eye. A similar sampling procedure was used in the LGNd of the two control animals. Recordings were made with extremely fine-tipped micropipettes (impedances = 50-80 M omega at 200 Hz when filled with 3M NaCl; tip diameters less than 0.3 micron when measured with scanning electron-microscopical methods). Micropipettes with these tip sizes were shown previously (Friedlander et al. 1981) to display no sampling bias on the basis of soma size in the LGNd of normal cats. Only data from complete penetrations through the non-deprived and deprived laminae were used in our analysis. Each animal's non-deprived lamina A also provided control data. In addition, we recorded from geniculocortical axons in the optic radiations above the LGNd (ipsilateral to the deprived eye in five cats and both ipsilateral and contralateral to the deprived eye in one cat). The percentages of X- and Y-cells encountered in the LGNd of our control animals is in agreement with previous estimates based on cell size (2:1 X- to Y-cells - Friedlander et al. 1981). In the present study, fewer normal X- and Y-cells were encountered in laminae innervated by the deprived eye than in laminae innervated by the non-deprived eye in every MD animal. When these values were normalized for the shrinkage of the deprived lamina, only the Y-cell population was significantly reduced. These differences are highly significant both when the data from all of the animals are pooled (chi 2 = 21.77; P less than 0.0001), and when the comparisons are made for individual animals (P less than 0.02, Mann-Whitney U-test). While some of the reduction in Y-cells may be due to an increase in the number of cells with abnormal receptive field properties, too few abnormal cells were encountered to totally account for the reduction in the number of Y-cells.(ABSTRACT TRUNCATED AT 400 WORDS)
6只猫从1周龄起就进行单眼眼睑缝合饲养。2只对照动物在同一群体中饲养。单眼剥夺（MD）猫的缝合眼在8 - 9个月龄时打开。通过细胞外记录剥夺眼同侧LGNd A层中单个神经元的活动，评估单眼剥夺对背外侧膝状核（LGNd）中神经元功能类别的分布的影响。在2只对照动物的LGNd中使用了类似的采样程序。用极细尖端的微电极进行记录（充满3M NaCl时，在200 Hz下阻抗为50 - 80 MΩ；用扫描电子显微镜方法测量时尖端直径小于0.3微米）。先前已表明（Friedlander等人，1981年），这种尖端尺寸的微电极在正常猫的LGNd中基于细胞大小不会表现出采样偏差。我们的分析仅使用完全穿透非剥夺层和剥夺层的数据。每只动物的非剥夺A层也提供对照数据。此外，我们在LGNd上方的视辐射中记录了膝状体皮质轴突（5只猫中是剥夺眼同侧，1只猫中是剥夺眼同侧和对侧）。我们对照动物LGNd中遇到的X细胞和Y细胞的百分比与先前基于细胞大小的估计一致（X细胞与Y细胞比例为2:1 - Friedlander等人，1981年）。在本研究中，在每只MD动物中，剥夺眼支配的层中遇到的正常X细胞和Y细胞比非剥夺眼支配的层中少。当这些值针对剥夺层的收缩进行归一化时，只有Y细胞群体显著减少。当汇总所有动物的数据时（卡方 = 21.77；P < 0.0001），以及对个体动物进行比较时（P < 0.02，曼 - 惠特尼U检验），这些差异都非常显著。虽然Y细胞数量的一些减少可能是由于具有异常感受野特性的细胞数量增加，但遇到的异常细胞太少，无法完全解释Y细胞数量的减少。（摘要截断于400字）
