Kratz K E, Webb S V, Sherman S M
J Comp Neurol. 1978 Oct 1;181(3):615-25. doi: 10.1002/cne.901810309.
Single unit extracellular recordings, cell size measurements, and cell packing density measurements were made in the medial interlaminar nucleus (MIN) of nine adult cats that had been monocularly deprived by lid suture prior to natural eye opening. The electrophysiological properties of neurons in the nondeprived regions of MIN (areas receiving input from the non-deprived eye) remained unaffected by monocular lid suture. The latencies to optic chiasm stimulation and receptive field properties, including receptive field center size, were essentially the same as those found for MIN neurons of normal adult cats. In contrast, cells in the deprived regions were severely affected by monocular deprivation. We encountered in the deprived regions of MIN only about one half as many active neurons per mm of electrode penetration as we did in the nondeprived regions. Of the physiologically active cells remaining, about one half had abnormal receptive field and/or response properties. This resulted in a sampling density of 5.1 normal Y-cells per mm of penetration in nondeprived regions of MIN compared to 1.0 normal Y-cell per mm in deprived regions of MIN. Histological effects of deprivation were also seen. Deprived regions of MIN were distinguished from nondeprived regions in four cats by autoradiography following intravitreal injection of tritiated proline into the deprived or non-deprived eye (2 cats each). The mean cell size of deprived regions of MIN was 34% smaller than that of nondeprived regions. We did not find a difference in cell packing density between these two regions. It appears that the effects of monocular lid suture upon MIN are in most respects similar to the effects of monocular lid suture previously reported for the A laminae. Since MIN is composed solely of Y-cells, these data support the idea that the Y-pathways are more severely affected by visual deprivation than are the X-pathways. Further, since MIN projects largely outside the striate cortex, these data give the first demonstration of a primary effect of early lid suture upon extrastriate visual pathways.
对9只成年猫的内侧层间核（MIN）进行了单单位细胞外记录、细胞大小测量和细胞堆积密度测量，这些猫在自然睁眼之前就已通过眼睑缝合进行了单眼剥夺。MIN未被剥夺区域（接受未被剥夺眼输入的区域）的神经元电生理特性未受单眼眼睑缝合的影响。对视交叉刺激的潜伏期和感受野特性，包括感受野中心大小，与正常成年猫MIN神经元的情况基本相同。相比之下，被剥夺区域的细胞受到单眼剥夺的严重影响。在MIN的被剥夺区域，每毫米电极穿刺所遇到的活跃神经元数量仅为未被剥夺区域的一半左右。在剩余的生理活跃细胞中，约一半具有异常的感受野和/或反应特性。这导致在MIN未被剥夺区域每毫米穿刺的正常Y细胞采样密度为5.1个，而在MIN被剥夺区域每毫米为1.0个正常Y细胞。还观察到了剥夺的组织学效应。在4只猫中，通过向被剥夺或未被剥夺眼玻璃体内注射氚标记的脯氨酸后进行放射自显影，区分了MIN的被剥夺区域和未被剥夺区域（每组2只猫）。MIN被剥夺区域的平均细胞大小比未被剥夺区域小34%。我们未发现这两个区域在细胞堆积密度上存在差异。看来单眼眼睑缝合对MIN的影响在大多数方面与先前报道的对A层的单眼眼睑缝合影响相似。由于MIN仅由Y细胞组成，这些数据支持了Y通路比X通路更容易受到视觉剥夺影响的观点。此外，由于MIN主要投射到纹状皮层之外，这些数据首次证明了早期眼睑缝合对纹外视觉通路的主要影响。
