Snyder C E, Nadziejko C E, Herp A
In Vitro. 1984 Feb;20(2):95-102. doi: 10.1007/BF02626649.
A serum-free culture medium was employed to evaluate both qualitative and quantitative aspects of secreted proteins derived from human bronchial explants over a period of 26 to 50 d. It was found that protein and hexose were secreted at a reasonably constant rate during this period. Viability of explants was assessed by histological examination, attachment to scored grids of the petri dish, incorporation of labeled precursors into tissue proteins, and by fluorographic analysis of the sodium dodecyl sulfate-polyacrylamide gel pattern of the secreted material. This culture system is useful as a model for the study of secretory products and processes and how they are affected by various stimuli.
采用无血清培养基在26至50天的时间内评估源自人支气管外植体的分泌蛋白的定性和定量方面。结果发现，在此期间蛋白质和己糖以相当恒定的速率分泌。通过组织学检查、外植体在培养皿刻痕网格上的附着情况、标记前体掺入组织蛋白以及对分泌物质的十二烷基硫酸钠-聚丙烯酰胺凝胶图谱进行荧光显影分析来评估外植体的活力。该培养系统作为研究分泌产物和过程以及它们如何受到各种刺激影响的模型很有用。
