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凝集素在人支气管黏膜腺细胞中用于检测糖缀合物的应用。

Use of lectins for detection of glycoconjugates in the glandular cells of the human bronchial mucosa.

作者信息

Mazzuca M, Lhermitte M, Lafitte J J, Roussel P

出版信息

J Histochem Cytochem. 1982 Sep;30(9):956-66. doi: 10.1177/30.9.7130674.

Abstract

Paraffin-embedded human bronchial biopsies, obtained in macroscopically healthy areas, were examined using nine peroxidase-bound lectins. These were either isolated or purified by affinity column chromatography (Ulex europeus, Triticum vulgare, Glycine max, and Arachis hypogatea lectins) or commercial preparations (Lotus tetragonolobus, Canavalia ensiformis, Helix pomatia, Phaseolus vulgaris, and Lens culinaris lectins) and conjugated to peroxidase (except for concanavalin A). These labeled lectins were used as specific molecular probes to localize differences in carbohydrate-containing components present in the different types of glandular cells of human bronchial mucosa. The choice of fixative was crucial and tests used for this study have shown that Carnoy's solution seems to be the most appropriate solution to preserve mucous glycoproteins in situ. Comparison of the affinity of several lectins for human bronchial glycoproteins and for bronchial mucosa demonstrates the predominance of serum-type glycoproteins in serous cells of the submucosal glands and mucin-type glycoproteins in mucous cells of the submucosal glands and in goblet cells of the bronchial epithelium. Furthermore the data obtained with some lectins, such as Helix pomatia agglutinin, suggest that there are some differences in the mucins synthesized by goblet cells and by mucous cells.

摘要

使用九种过氧化物酶结合凝集素对取自肉眼可见健康区域的石蜡包埋人支气管活检组织进行检查。这些凝集素要么通过亲和柱色谱法分离或纯化(欧洲荆豆、普通小麦、大豆和落花生凝集素),要么是商业制剂(四角豆、刀豆、圆田螺、菜豆和小扁豆凝集素),并与过氧化物酶偶联(伴刀豆球蛋白A除外)。这些标记的凝集素用作特异性分子探针,以定位人支气管黏膜不同类型腺细胞中含碳水化合物成分的差异。固定剂的选择至关重要,本研究使用的测试表明,卡诺氏液似乎是原位保存黏液糖蛋白最合适的溶液。几种凝集素对人支气管糖蛋白和支气管黏膜亲和力的比较表明,血清型糖蛋白在黏膜下腺的浆液细胞中占优势,而黏蛋白型糖蛋白在黏膜下腺的黏液细胞和支气管上皮的杯状细胞中占优势。此外,用一些凝集素(如圆田螺凝集素)获得的数据表明,杯状细胞和黏液细胞合成的黏蛋白存在一些差异。

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