McCaskill A C, Hosking C S, Hill D J
Immunology. 1984 Apr;51(4):669-77.
Mice sensitized with two intraperitoneal injections of ovalbumin and challenged intranasally with the same antigen developed a non-fatal anaphylactic shock peaking in severity 30 min after challenge. Increases in haematocrit were noted which corresponded to the severity of signs of shock displayed by mice. Severity of shock also correlated with IgE and IgG levels. Sensitization by the nasal route, and use of B. pertussis vaccine as adjuvant had no qualitative effect upon the response. Cobra venom factor depletion of C3 in vivo did not alter the response of mice, which suggests anaphylaxis did not involve complement activation. Sensitivity was not transferrable to non-immune mice with serum. Passive sensitization with polyclonal and monoclonal antibodies produced inconsistent results. Possible mechanisms of anaphylaxis are discussed.
通过腹腔内注射两次卵清蛋白致敏的小鼠，再经鼻内注射相同抗原进行激发，会引发非致命性过敏休克，休克严重程度在激发后30分钟达到峰值。观察到血细胞比容升高，这与小鼠表现出的休克体征严重程度相对应。休克严重程度也与IgE和IgG水平相关。经鼻途径致敏以及使用百日咳杆菌疫苗作为佐剂对反应没有定性影响。体内用眼镜蛇毒因子消耗C3并没有改变小鼠的反应，这表明过敏反应不涉及补体激活。敏感性不能通过血清转移给非免疫小鼠。用多克隆和单克隆抗体进行被动致敏产生的结果不一致。文中讨论了过敏反应的可能机制。
