Dahlbäck M, Bergstrand H, Pauwels R, Bazin H
Allergy. 1983 May;38(4):261-71. doi: 10.1111/j.1398-9995.1983.tb01619.x.
SD rats were sensitized by an i.p. injection of 1 microgram or 10 micrograms ovalbumin (OA) together with 10 mg Silica gel. At the indicated time after sensitization, allergic response capacity of the animals was estimated by measuring changes in intratracheal pressure induced by an i.v. injection of 0.3 mg OA (low challenge dose) or 5 mg OA (high challenge dose). Animals given 1 microgram OA in Silica gel showed no response capacity at 14, 35 or 47-48 days after sensitization. Animals injected with 10 micrograms OA showed a clear-cut response with a peak at 14 days; at 7 weeks after immunization the response capacity had faded almost completely. Specific OA-IgE antibody and total IgE serum levels were examined by radioimmunoassay. A slight increase in OA-IgE antibody was recorded in animals injected 14 days before test with 10 micrograms ovalbumin and Silica gel; animals given 1 microgram OA and Silica showed no OA-IgE antibody. Five weeks after sensitization, some animals of each group were injected i.p. with 100 mg alum, B. pertussis vaccine (2 ml of Perthydral), or saline, all injections being made without any further antigen addition. 12-13 days after such an injection, alum-treated animals showed high response capacity at bronchial anaphylactic tests and high OA-IgE antibody levels but comparably low levels of total IgE. Animals injected with B. pertussis, on the other hand, showed low response capacity (bronchial anaphylactic tests and OA-IgE antibody levels) but high total IgE-antibody levels. The bronchial anaphylactic response in the B. pertussis-injected animals but not the alum-injected animals was significantly correlated to serum IgG2a antibody levels. These results show that injection of alum or B. pertussis vaccine without antigen can precipitate/enhance anaphylactic response capacity and production of specific and non-specific IgE and IgG2a.
将1微克或10微克卵清蛋白（OA）与10毫克硅胶通过腹腔注射对SD大鼠进行致敏。在致敏后的指定时间，通过测量静脉注射0.3毫克OA（低激发剂量）或5毫克OA（高激发剂量）所诱导的气管内压力变化来评估动物的过敏反应能力。用硅胶给予1微克OA的动物在致敏后14、35或47 - 48天没有反应能力。注射10微克OA的动物在14天出现明显反应且达到峰值；免疫7周后反应能力几乎完全消失。通过放射免疫测定法检测特异性OA - IgE抗体和总IgE血清水平。在试验前14天用10微克卵清蛋白和硅胶注射的动物中记录到OA - IgE抗体略有增加；给予1微克OA和硅胶的动物未显示OA - IgE抗体。致敏5周后，每组的一些动物腹腔注射100毫克明矾、百日咳疫苗（2毫升Perthydral）或生理盐水，所有注射均不添加任何进一步的抗原。在这种注射后12 - 13天，明矾处理的动物在支气管过敏试验中显示出高反应能力和高OA - IgE抗体水平，但总IgE水平相对较低。另一方面，注射百日咳杆菌的动物显示出低反应能力（支气管过敏试验和OA - IgE抗体水平）但总IgE - 抗体水平高。注射百日咳杆菌的动物而非注射明矾的动物的支气管过敏反应与血清IgG2a抗体水平显著相关。这些结果表明，无抗原注射明矾或百日咳疫苗可引发/增强过敏反应能力以及特异性和非特异性IgE和IgG2a的产生。
