Earnshaw W C, Halligan N, Cooke C, Rothfield N
J Cell Biol. 1984 Jan;98(1):352-7. doi: 10.1083/jcb.98.1.352.
We used antisera from patients with the CREST syndrome of scleroderma (calcinosis, Raynaud's phenomenon, esophageal dysmotility, sclerodactyly, telangiectasia) to show that an antigenic component of the kinetochore present in metaphase chromosomes is also present in nonhistone chromosome scaffolds isolated following extensive digestion of the DNA and extraction of the bulk of chromosomal protein. All sera from 12 scleroderma CREST patients previously shown by immunofluorescence microscopy to have circulating antikinetochore antibodies recognise a protein of Mr 77,000 (CREST-77) in an immunoblotting assay. 9 of the 12 sera also recognise an antigen of Mr 110,000 (CREST-110). These proteins are present in isolated chromosomes and nonhistone scaffolds derived from them by two different procedures. Sera of five scleroderma CREST patients who are antikinetochore negative (by immunofluorescence) bind to neither protein in immunoblots. These data suggest that CREST-77 (and possibly CREST-110) is a component of the human kinetochore, and that the kinetochore is an integral part of the mitotic chromosome scaffolding.
我们使用了硬皮病CREST综合征（钙质沉着、雷诺现象、食管运动障碍、指端硬化、毛细血管扩张）患者的抗血清，以表明中期染色体中存在的动粒抗原成分，在经过DNA大量消化和大部分染色体蛋白提取后分离得到的非组蛋白染色体支架中也存在。先前通过免疫荧光显微镜检查显示具有循环抗动粒抗体的12例硬皮病CREST患者的所有血清，在免疫印迹试验中均识别出一种分子量为77,000的蛋白质（CREST - 77）。12例血清中的9例还识别出一种分子量为110,000的抗原（CREST - 110）。这些蛋白质存在于通过两种不同方法从其衍生而来的分离染色体和非组蛋白支架中。5例抗动粒阴性（通过免疫荧光）的硬皮病CREST患者的血清在免疫印迹中均不与这两种蛋白质结合。这些数据表明CREST - 77（可能还有CREST - 110）是人类动粒的一个成分，并且动粒是有丝分裂染色体支架的一个组成部分。
