Eberle R, Hilliard J K
J Virol. 1984 May;50(2):316-24. doi: 10.1128/JVI.50.2.316-324.1984.
The replication of the simian herpesvirus SA8 in Vero cells was examined. The time course of replication of the simian herpesvirus SA8 was found to be similar to that of the herpes simplex viruses. Infectious progeny virions were first detectable by 6 h postinfection and were readily released into the extracellular fluids beginning at 9 h postinfection. All cell lines tested, with the exception of Madin-Darby canine kidney cells, were permissive for SA8. Analysis of SA8-infected cells by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed over 40 infected cell polypeptides ranging in molecular weight from 158,000 to less than 10,000. Of these proteins, 23 were present in virions. Three classes of infected cell polypeptides could be identified based on the kinetics of their synthesis. Post-translational processing of several SA8-induced proteins was also observed in pulse-chase experiments. Six distinct SA8-specific glycoproteins ranging from 118,000 to 19,500 daltons were also identified in infected cells. Of these glycoproteins, five were present in virions.
对猿猴疱疹病毒SA8在非洲绿猴肾细胞中的复制进行了检测。发现猿猴疱疹病毒SA8的复制时间进程与单纯疱疹病毒相似。感染后6小时首次检测到有感染性的子代病毒粒子，从感染后9小时开始，这些病毒粒子很容易释放到细胞外液中。除了犬肾细胞系外，所有测试的细胞系对SA8均具有易感性。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳对感染SA8的细胞进行分析，结果显示有40多种感染细胞多肽，其分子量范围从158,000到小于10,000。在这些蛋白质中，有23种存在于病毒粒子中。根据合成动力学可以鉴定出三类感染细胞多肽。在脉冲追踪实验中还观察到了几种SA8诱导蛋白的翻译后加工过程。在感染细胞中还鉴定出了六种不同的SA8特异性糖蛋白，分子量从118,000到19,500道尔顿不等。在这些糖蛋白中，有五种存在于病毒粒子中。
