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猴疱疹病毒(B病毒):病毒在感染细胞中的复制及病毒多肽的鉴定

Herpesvirus simiae (B virus): replication of the virus and identification of viral polypeptides in infected cells.

作者信息

Hilliard J K, Eberle R, Lipper S L, Munoz R M, Weiss S A

出版信息

Arch Virol. 1987;93(3-4):185-98. doi: 10.1007/BF01310973.

DOI:10.1007/BF01310973
PMID:3030236
Abstract

The events and products of replication of Herpesvirus simiae (B virus) in Vero cells were studied. The time course of the synthetic events of DNA replication and protein synthesis were found to be similar to the processes of the herpes simplex viruses and SA 8. Infectious progeny virus were detected by 4 hours post infection and were first found extracellularly between 6 and 8 hours post infection (PI). As in the case of SA 8, all cell lines tested were permissive for lytic infection by B virus. Analyses of B virus-infected cells by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) revealed approximately 50 infected cell polypeptides (ICP) ranging in molecular weight from about 26,000 to 239,000 daltons. The kinetics of synthesis of the ICPs were also identified. At least nine glucosamine-containing glycopeptides were noted ranging from 133,000 to 29,000 daltons.

摘要

研究了猴疱疹病毒(B病毒)在非洲绿猴肾细胞中的复制事件和产物。发现DNA复制和蛋白质合成的合成事件的时间进程与单纯疱疹病毒和SA 8的过程相似。感染后4小时检测到感染性子代病毒,首次在感染后6至8小时在细胞外发现。与SA 8的情况一样,所有测试的细胞系对B病毒的裂解感染均具有易感性。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)对B病毒感染的细胞进行分析,发现约50种感染细胞多肽(ICP),分子量范围约为26,000至239,000道尔顿。还确定了ICP的合成动力学。注意到至少九种含葡萄糖胺的糖肽,分子量范围为133,000至29,000道尔顿。

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