van Steenbrugge G J, Groen M, Romijn J C, Schröder F H
J Urol. 1984 Apr;131(4):812-7. doi: 10.1016/s0022-5347(17)50630-8.
The effects of hormonal manipulation on the growth of a transplantable human prostatic carcinoma line (PC-82) were studied. The histological pattern of the PC-82 tumor, which still closely resembles the original tumor material, and the tumor growth rate did not change during the subsequent mouse passages. Growth of PC-82 tumor tissue on female and castrated male mice did not occur. Castration of tumor-bearing mice resulted in a cessation of tumor growth, after which the tumor volume decreased 50 +/- 27 per cent within 6 weeks after castration. Hormone-independent regrowth of the tumor tissue was not observed after long-term withdrawal of androgens. After a period of 10 weeks following tumor growth arrest, administration of testosterone almost directly resulted in regrowth of the tumor. Hormones, testosterone and estradiol, were administered by silastic implants. Intact male nude mice were shown to have highly fluctuating levels of testosterone. Implantation with testosterone resulted in constant levels of circulating testosterone, which could be maintained for at least 10 weeks, while the mean concentration of plasma testosterone was not different from that in control male mice. The doubling time of tumors grown on testosterone-substituted intact female and intact and castrated male mice was significantly shorter than that of tumors grown on intact male mice. Histologically the tumors grown on androgen-substituted mice were similar to those grown on untreated mice; the mitotic index, however, was much higher in the testosterone treated animals. Implantation of intact male mice with estradiol suppressed plasma testosterone to a mean level of 1 ng. per ml. and prevented the growth of PC-82 tumor tissue almost completely. Treatment of tumor-bearing mice with an estradiol implant following androgen withdrawal did not result in a further decrease of the tumor volume compared to the mice without additional estradiol implantation.
研究了激素调控对一种可移植的人前列腺癌细胞系（PC - 82）生长的影响。PC - 82肿瘤的组织学模式仍与原始肿瘤材料极为相似，且在随后的小鼠传代过程中肿瘤生长速率未发生改变。PC - 82肿瘤组织在雌性和去势雄性小鼠身上不生长。对荷瘤小鼠进行去势导致肿瘤生长停止，去势后6周内肿瘤体积减小50±27%。长期撤除雄激素后未观察到肿瘤组织的激素非依赖性再生。在肿瘤生长停止10周后，给予睾酮几乎直接导致肿瘤再生。激素、睾酮和雌二醇通过硅橡胶植入物给药。完整雄性裸鼠的睾酮水平波动很大。植入睾酮导致循环睾酮水平恒定，可维持至少10周，而血浆睾酮的平均浓度与对照雄性小鼠无异。在睾酮替代的完整雌性、完整和去势雄性小鼠身上生长的肿瘤倍增时间显著短于在完整雄性小鼠身上生长的肿瘤。组织学上，在雄激素替代小鼠身上生长的肿瘤与未处理小鼠身上生长的肿瘤相似；然而，在睾酮处理的动物中，有丝分裂指数要高得多。给完整雄性小鼠植入雌二醇可将血浆睾酮抑制至平均每毫升1纳克的水平，并几乎完全阻止PC - 82肿瘤组织的生长。与未额外植入雌二醇相比，雄激素撤除后用雌二醇植入物处理荷瘤小鼠并未导致肿瘤体积进一步减小。
