Folkerd E J, James V H
J Steroid Biochem. 1984 Feb;20(2):679-81. doi: 10.1016/0022-4731(84)90143-2.
Using slices of human adipose tissue maintained in medium 199 for 40 h at 37 degrees C, it was observed that in the absence of any other additions, the presence of foetal calf serum (10%) inhibits basal levels of aromatase activity. Including dexamethasone (1 X 10(-7) M) in the medium increases aromatase activity approximately 10-fold in the presence of foetal calf serum but has only a small stimulatory effect in its absence. The combined addition of dexamethasone (1 X 10(-7) M) and human prolactin (0.8 ng/ml) has a marked additional incremental effect in the presence or absence of foetal calf serum. In contrast, the addition of prolactin to the medium without glucocorticoid did not stimulate oestrogen production. The possibility that prolactin could be the substance in foetal calf serum responsible for mediating the stimulatory effect of glucocorticoids on oestrogen production is considered.
使用在37℃下于199培养基中保存40小时的人脂肪组织切片，观察到在没有任何其他添加物的情况下，胎牛血清（10%）的存在会抑制芳香化酶活性的基础水平。在培养基中加入地塞米松（1×10⁻⁷ M），在有胎牛血清存在时可使芳香化酶活性增加约10倍，但在无胎牛血清时仅有很小的刺激作用。在有或无胎牛血清的情况下，联合加入地塞米松（1×10⁻⁷ M）和人催乳素（0.8 ng/ml）具有显著的额外增量效应。相比之下，在没有糖皮质激素的培养基中加入催乳素不会刺激雌激素的产生。考虑了催乳素可能是胎牛血清中负责介导糖皮质激素对雌激素产生刺激作用的物质这一可能性。
