作为Cot参数的DNA浓度（Co）和孵育时间（t）是否会影响DNA双链体的热稳定性？

Does the concentration of DNA (Co) and the time of incubation (t) as parameters of Cot influence the thermal stability of the DNA duplexes?

作者信息

Bachvarov D R, Ivanov I G, Markov G G

出版信息

Mol Biol Rep. 1984 Jan;9(4):223-6. doi: 10.1007/BF00775351.

Abstract

It has been shown in a previous paper (8) that the prime product of reassociation of related DNA sequences under open experimental conditions are mismatched duplexes which undergo 'maturation' upon further incubation. Due to this feature, the Tm value of the duplexes of a large number of DNAs is strongly dependent on the Cot value. Here we present data showing that the Tm of the duplexes of such type of DNAs depends also on the concentration of DNA in the range of one and the same Cot value. The significance of this finding in studying the taxonomic relationship by DNA-DNA hybridisation is discussed.

摘要

在之前的一篇论文（8）中已经表明，在开放实验条件下相关DNA序列重新缔合的主要产物是错配双链体，其在进一步孵育时会经历“成熟”。由于这一特性，大量DNA双链体的解链温度（Tm值）强烈依赖于Cot值。在此，我们给出的数据表明，在相同的Cot值范围内，这类DNA双链体的Tm值也取决于DNA的浓度。本文讨论了这一发现对于通过DNA-DNA杂交研究分类学关系的意义。