Ivanov I, Antanov P, Markova N, Markov G
Mol Biol Rep. 1978 Jun 16;4(2):67-71. doi: 10.1007/BF00775962.
Reassociation of typical single-copy DNAs, like E. coli DNA, even when performed at relatively low temperatures, results in the formation of perfect duplexes with thermal stability very close to that of the native DNA. In contrast, duplexes of mouse repeated DNA as well as duplexes of Streptomyces DNA prepared under the same conditions, show a low thermal stability and undergo post-reassociation changes upon prolonged incubation. These changes, called "maturation" of the DNA duplexes, result in increasing of their thermal stability. Some of the factors affecting the rate of "maturation" are studied. The implication of the "maturation" process in reassociation analysis and in characterization of the heterogeneity of DNA is discussed.
典型的单拷贝DNA（如大肠杆菌DNA）重新退火，即使在相对较低的温度下进行，也会形成具有与天然DNA非常接近的热稳定性的完美双链体。相比之下，在相同条件下制备的小鼠重复DNA双链体以及链霉菌DNA双链体，热稳定性较低，并且在长时间孵育后会发生重新退火后的变化。这些变化被称为DNA双链体的“成熟”，会导致其热稳定性增加。研究了一些影响“成熟”速率的因素。讨论了“成熟”过程在重新退火分析和DNA异质性表征中的意义。
