通过重缔合动力学对纤细裸藻DNA的表征。

The characterization of Euglena gracilis DNA by its reassociation kinetics.

作者信息

Rawson J R

出版信息

Biochim Biophys Acta. 1975 Aug 21;402(2):171-8. doi: 10.1016/0005-2787(75)90036-2.

Abstract

The reassociation kinetics of Euglena gracilis DNA were carried out to determine the Cot 1/2 of the unique DNA (2000). The Cot 1/2 of the unique DNA and the amount of DNA per cell were used to show that this alga is not polyploid. Three kinetically definable classes of DNA were observed: a highly repetitive fraction, a middle repetitive fraction, and a non-repetitive fraction. Each fraction of DNA was characterized by its melting properties (Tm).

摘要

进行纤细裸藻DNA的复性动力学研究以确定其单拷贝DNA的Cot 1/2值（2000）。利用单拷贝DNA的Cot 1/2值和每个细胞的DNA量来表明这种藻类不是多倍体。观察到三类动力学上可定义的DNA：高度重复部分、中度重复部分和非重复部分。每部分DNA都通过其解链特性（熔解温度）进行表征。