Conley F K
Neurosurgery. 1984 Feb;14(2):187-92. doi: 10.1227/00006123-198402000-00012.
This study reports a model of metastatic tumor to the brains of mice. A sequentially colonized brain-homing tumor line was developed from the KHT sarcoma and was injected into the right hind foot of syngeneic C3H female mice. When tumor was just grossly apparent in the foot, the tumor-bearing leg was amputated at the midthigh level, thus surgically curing each mouse of its primary tumor. Mice were followed to death and were autopsied for evidence of metastatic tumor. All mice had metastatic pulmonary tumor nodules, and approximately 80% of the mice had metastatic deposits of tumor in the brain. Because this model mimics the situation in humans where patients die of metastatic disease at some time after the removal of their primary cancer, it should be of value in further delineating factors that influence the development and growth of metastatic tumor in the central nervous system.
本研究报告了一种小鼠脑转移瘤模型。从KHT肉瘤中培育出一种依次定殖的脑归巢肿瘤细胞系，并将其注射到同基因C3H雌性小鼠的右后足。当足部肿瘤肉眼刚可辨时，在大腿中部水平截断荷瘤腿，从而通过手术治愈每只小鼠的原发性肿瘤。对小鼠进行跟踪直至死亡，并进行尸检以寻找转移瘤的证据。所有小鼠均有转移性肺肿瘤结节，约80%的小鼠脑中有肿瘤转移灶。由于该模型模拟了人类患者在原发性癌症切除后的某个时间死于转移性疾病的情况，因此在进一步阐明影响中枢神经系统转移性肿瘤发生和生长的因素方面应具有价值。
