Newman J F, Rowlands D J, Brown F, Goodridge D, Burrows R, Steck F
Intervirology. 1977;8(3):145-54. doi: 10.1159/000148889.
The physicochemical properties of two serologically distinct equine rhinoviruses have been examined. Each virus sedimented at approximately 160S but co-centrifugation of the two viruses in a sucrose gradient revealed a small difference in their sedimentation coefficients. The two viruses also have different buoyant densities in cesium chloride. The equine rhinovirus type 1 equilibrated as a sharp peak at 1.45 g/ml whereas the type 2 virus equilibrated as a heterogeneous band with a peak at 1.44 g/ml but ranging in density from 1.41 to 1.45 g/ml. The relative sedimentation coefficients of the two virus RNAs were 35S for rhinovirus 1 and 37S for rhinovirus 2. A limited number of base composition analyses also showed differences between the two virus RNAs. The polypeptide profile of each serotype in polyacrylamide gels was generally similar to those of other picornaviruses but the two serotypes could be distinguished readily from each other.
对两种血清学上不同的马鼻病毒的理化特性进行了研究。每种病毒在约160S处沉降，但将这两种病毒在蔗糖梯度中共同离心显示它们的沉降系数存在微小差异。这两种病毒在氯化铯中的浮力密度也不同。1型马鼻病毒在1.45 g/ml处平衡为一个尖锐峰，而2型病毒平衡为一个密度不均一的条带，峰密度在1.44 g/ml，密度范围为1.41至1.45 g/ml。两种病毒RNA的相对沉降系数分别为1型鼻病毒35S和2型鼻病毒37S。有限数量的碱基组成分析也显示两种病毒RNA之间存在差异。每种血清型在聚丙烯酰胺凝胶中的多肽图谱通常与其他小RNA病毒相似，但这两种血清型很容易相互区分。
