Bashiruddin J B, Martin J L, Reinganum C
Department of Microbiology, Monash University Medical School, Prahran, Victoria, Australia.
Arch Virol. 1988;100(1-2):61-74. doi: 10.1007/BF01310908.
A picornavirus was isolated from various life stages of the Queensland fruit fly, Dacus tryoni. This virus, Queensland fruit fly virus (QFFV) has virions with a diameter of 30 nm and a sedimentation coefficient of 178 S. One third of the particles in preparations were empty capsids or natural top component (NTC) with a sedimentation coefficient of 95 S. The buoyant density (rho) of virions and NTC in CsCl was 1.34 and 1.30 g/ml respectively; small amounts of a dense component (rho = 1.45 g/ml) were also detected. The capsid contained three major protein species of molecular weight (mol.wt.) 41,700, 36,500, and 31,300, in approximately equimolar proportions. NTC contained three major species of mol. wt. 44,700, 41,700, and 31,300. The nucleic acid present only in the bottom component virions was RNA and comprised about 30% of the particle weight and had a mol. wt of 2.88 kd, contained a poly(A) tract, and had a base ratio: G = 20; A = 32; C = 15; U = 33. The mol. wt. of the virion was estimated to be approximately equal to 9.5 kd. When virions were heated at 56 degrees C and above, they converted into artificial top component (ATC), which had the same protein composition as the virion when analysed by SDS-PAGE. In immunodiffusion tests the virions and NTC were indistinguishable, but a minor difference in antigenicity was detected between the virions and ATC. Virions were stable between pH 3 and 9 inclusive, and between 5 and 7 in the presence of 0.14 M NaCl. Immunodiffusion tests showed that QFFV was serologically unrelated to a range of picornaviruses as well as an unclassified virus isolated from the Mediterranean fruit fly, Ceratitis capitata. The data show that QFFV is probably a member of the Picornaviridae, genus Enterovirus.
从昆士兰果蝇（Dacus tryoni）的不同生命阶段分离出一种小核糖核酸病毒。这种病毒，即昆士兰果蝇病毒（QFFV），其病毒粒子直径为30纳米，沉降系数为178 S。制剂中三分之一的粒子为空衣壳或天然顶部成分（NTC），沉降系数为95 S。在氯化铯中，病毒粒子和NTC的浮力密度（ρ）分别为1.34和1.30克/毫升；还检测到少量密度较大的成分（ρ = 1.45克/毫升）。衣壳包含三种主要蛋白质，分子量分别为41,700、36,500和31,300，比例大致等摩尔。NTC包含分子量分别为44,700、41,700和31,300的三种主要成分。仅存在于底部成分病毒粒子中的核酸是RNA，约占粒子重量的30%，分子量为2.88千道尔顿，含有一个聚腺苷酸序列，碱基比例为：G = 20；A = 32；C = 15；U = 33。病毒粒子的分子量估计约为9.5千道尔顿。当病毒粒子在56摄氏度及以上加热时，它们会转化为人工顶部成分（ATC），通过SDS - PAGE分析，其蛋白质组成与病毒粒子相同。在免疫扩散试验中，病毒粒子和NTC无法区分，但在病毒粒子和ATC之间检测到了抗原性的微小差异。病毒粒子在pH值3至9（含）之间以及在0.14 M氯化钠存在下pH值5至7之间稳定。免疫扩散试验表明，QFFV在血清学上与一系列小核糖核酸病毒以及从地中海实蝇（Ceratitis capitata）分离出的一种未分类病毒无关。数据表明，QFFV可能是肠道病毒属小核糖核酸病毒科的成员。
