Sarto G E, Stubblefield P A, Lurain J, Therman E
Am J Obstet Gynecol. 1984 Apr 1;148(7):1014-23. doi: 10.1016/0002-9378(84)90545-3.
Nuclear morphology and DNA synthesis were analyzed to determine the mechanism through which hydatidiform moles proliferate. Hydatidiform moles are characterized by a great variation in nuclear morphology and size. There are cells with small nuclei of variable size that have chromocenters and Barr bodies which do not undergo DNA synthesis or mitosis, as well as cells in the diploid range that have evenly stained nuclei that display numerous mitoses and a high proportion of interphase nuclei in the process of DNA synthesis. Nuclei in the medium range show classical endomitotic stages. Endomitotic nuclei in endometaphase do not label with tritiated thymidine; endoanaphase nuclei may have one or a few chromosomes labeled, and endotelophase nuclei are heavily labeled. Nuclei that are evenly stained and are in the medium- to giant-size range label differently, depending upon their size. Many of the medium-sized nuclei are labeled, indicating DNA synthesis; the large nuclei are rarely labeled, and the giant nuclei are never labeled. The growth of a hydatidiform mole appears to be the result of normal mitosis and cytokinesis, as well as polyploidization and accompanying cell enlargement achieved through endomitosis and endoreduplication.
分析细胞核形态和DNA合成，以确定葡萄胎增殖的机制。葡萄胎的特征是细胞核形态和大小差异很大。存在细胞核小且大小不一的细胞，这些细胞核有染色中心和巴氏小体，不进行DNA合成或有丝分裂，还有二倍体范围内的细胞，其细胞核染色均匀，显示出大量有丝分裂，且在DNA合成过程中有高比例的间期核。中等大小的细胞核显示出典型的核内有丝分裂阶段。核内中期的核内有丝分裂细胞核不被氚标记的胸腺嘧啶核苷标记；核内后期的细胞核可能有一条或几条染色体被标记，而核内末期的细胞核被大量标记。染色均匀且大小在中等至巨大范围的细胞核标记情况不同，取决于它们的大小。许多中等大小的细胞核被标记，表明有DNA合成；大细胞核很少被标记，巨大细胞核从未被标记。葡萄胎的生长似乎是正常有丝分裂和胞质分裂的结果，以及通过核内有丝分裂和核内复制实现的多倍体化及伴随的细胞增大的结果。
