Kuhn E M, Therman E
Department of Medical Genetics, University of Wisconsin, Madison 53706.
Cancer Genet Cytogenet. 1988 Aug;34(1):143-51. doi: 10.1016/0165-4608(88)90181-1.
The arrangement of heterochromatin in various human and mouse nuclei has been analyzed with C banding. In most nuclei of 7-day mouse trophoblast, the heterochromatin consists of twin dots, or bigger clumps, apparently attached to the nuclear membrane. This finding agrees with the observation that most of these nuclei, which range from diploid to highly polyploid, show endomitotic stages. No polarization of heterochromatin in a Rabl orientation is seen in the trophoblast nuclei. Neither is a Rabl orientation found in the interphases of cultured human lymphocytes or fibroblasts. From their telophase arrangement, the chromosomes have obviously spread rapidly around the nuclear membrane. In many of the giant mouse trophoblast cells in vivo and in vitro, heterochromatin is apparently underreplicated. The same is true of giant cells in human hydatidiform moles and cervical cancer. Of the 82 cervical cancers analyzed, 46 showed chromocenters, and each tumor was characterized by its own pattern of heterochromatin.
已用C带分析法分析了各种人类和小鼠细胞核中异染色质的排列情况。在7日龄小鼠滋养层的大多数细胞核中，异染色质由双点或更大的团块组成，显然附着于核膜。这一发现与以下观察结果一致，即这些细胞核大多处于从二倍体到高度多倍体的状态，呈现核内有丝分裂阶段。在滋养层细胞核中未观察到异染色质以拉布尔（Rabl）方向极化。在培养的人类淋巴细胞或成纤维细胞的间期也未发现拉布尔方向。从末期排列来看，染色体显然已迅速围绕核膜散开。在许多体内和体外的巨大小鼠滋养层细胞中，异染色质明显复制不足。人类葡萄胎和宫颈癌中的巨细胞也是如此。在分析的82例宫颈癌中，46例显示有染色中心，且每个肿瘤都有其自身的异染色质模式。
