Kennedy W R, Sakuta M
Ann Neurol. 1984 Jan;15(1):73-8. doi: 10.1002/ana.410150114.
The collateral reinnervation of mouse sweat glands has been studied by a method that allows serial evaluation of the course of reinnervation in intact animals. The method is based on the finding that the activation of secretion from newly denervated sweat glands by pilocarpine or nerve stimulation is completely absent seven days after nerve section but returns with reinnervation. These characteristics allowed serial detection of footpad sweat glands newly reinnervated by collateral sprouting of the remaining intact saphenous nerve after section of the sciatic nerve. The number of saphenous innervated glands increased five- to sevenfold and the total saphenous sweat territory was greatly expanded across the volar surface of the hind paw. There was less enlargement when the sciatic nerve was allowed to regenerate and participate in reinnervation of sweat glands. None of the reinnervated glands were dually innervated by saphenous and sciatic nerves, as is the case in normal glands. When the saphenous nerve was sectioned after saphenous collateral sprouting was complete and after sciatic regeneration had innervated an apparent maximal number of glands, the sciatic nerve reacted by advancing farther into the formerly enlarged saphenous territory and re-reinnervated many of the glands. Collateral sprouting by sudomotor axons is more abundant and more widely dispersed than reported for larger nerve fibers to skeletal muscle and to low-threshold mechanoreceptors. It more closely resembles sprouting of nociceptive axons.
通过一种能对完整动物的神经再支配过程进行系列评估的方法，对小鼠汗腺的侧支神经再支配进行了研究。该方法基于以下发现：在神经切断后7天，毛果芸香碱或神经刺激对新去神经支配的汗腺分泌的激活作用完全消失，但随着神经再支配而恢复。这些特性使得在坐骨神经切断后，能够对由剩余完整的隐神经侧支发芽新再支配的足垫汗腺进行系列检测。隐神经支配的腺体数量增加了五到七倍，并且隐神经总的汗腺分布区域在后爪掌面显著扩大。当允许坐骨神经再生并参与汗腺的再支配时，扩大程度较小。与正常腺体不同，再支配的腺体没有一个同时接受隐神经和坐骨神经的双重支配。当隐神经侧支发芽完成且坐骨神经再生支配了明显最大数量的腺体后切断隐神经时，坐骨神经会进一步向先前扩大的隐神经分布区域延伸，并对许多腺体再次进行神经支配。与支配骨骼肌和低阈值机械感受器的较大神经纤维相比，汗腺运动轴突侧支发芽更丰富且分布更广泛。它更类似于伤害性轴突的发芽。
