更多证据表明野生二粒小麦是小麦B基因组的供体。

Additional evidence implicating Triticum searsii as the B-genome donor to wheat.

作者信息

Nath J, Hanzel J J, Thompson J P, McNay J W

出版信息

Biochem Genet. 1984 Feb;22(1-2):37-50. doi: 10.1007/BF00499285.

Abstract

In vitro DNA:DNA hybridizations and hydroxyapatite thermal-elution chromatography were employed to identify the diploid wheat species ancestral to the B genome of Triticum turgidum. 3H-T. turgidum DNA was hybridized to the unlabeled DNAs of T. urartu, T. speltoides, T. sharonensis, T. bicorne, T. longissimum, and T. searsii. 3H-Labeled DNAs of T. monococcum and a synthetic tetraploid AADD were hybridized with unlabeled DNAs of T. urartu and T. searsii to determine the relationship of the A genome of polyploid wheat and T. urartu. The heteroduplex thermal stabilities indicated that T. searsii was most closely related to the B genome of T. turgidum (AB) and that the genome of T. urartu and the A genome have a great deal of base-sequence homology. Thus, it appears that T. searsii is the B-genome donor to polyploid wheat or a major chromosome donor if the B genome is polyphyletic in origin.

摘要

采用体外DNA:DNA杂交和羟基磷灰石热洗脱色谱法来鉴定普通小麦B基因组的二倍体祖先物种。用3H标记的普通小麦DNA与乌拉尔图小麦、斯卑尔脱小麦、沙伦西小麦、二角小麦、长穗小麦和西尔斯小麦的未标记DNA进行杂交。用3H标记的一粒小麦DNA和合成四倍体AADD与乌拉尔图小麦和西尔斯小麦的未标记DNA进行杂交，以确定多倍体小麦A基因组与乌拉尔图小麦的关系。异源双链体的热稳定性表明，西尔斯小麦与普通小麦（AB）的B基因组关系最为密切，乌拉尔图小麦的基因组与A基因组有大量的碱基序列同源性。因此，似乎西尔斯小麦是多倍体小麦的B基因组供体，或者如果B基因组起源于多系，则是主要的染色体供体。