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High affinity specific antiestrogen binding sites are concentrated in rough microsomal membranes of rat liver.

作者信息

Watts C K, Sutherland R L

出版信息

Biochem Biophys Res Commun. 1984 Apr 16;120(1):109-15. doi: 10.1016/0006-291x(84)91420-7.

DOI:10.1016/0006-291x(84)91420-7
PMID:6712683
Abstract

Saturation and competitive binding analyses demonstrated the presence of a high affinity (KD = 0.92 nM), specific antiestrogen binding site (AEBS) in rat liver microsomes and at least 75% of total liver AEBS was recovered in this fraction. When microsomes were further separated into smooth and rough fractions, AEBS was concentrated in the latter. Subsequent dissociation of ribosomes from the rough membranes revealed that AEBS was associated with the membrane and not the ribosomal fraction. Antiestrogen binding activity could not be extracted from membranes with 1 M KCl or 0.5 M acetic acid but could be solubilized with sodium cholate. These data indicate that AEBS is an integral membrane component of the rough microsomal fraction of rat liver.

摘要

相似文献

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