正常泪液蛋白质谱及与年龄相关的变化。
Normal tear protein profiles and age-related changes.
作者信息
McGill J I, Liakos G M, Goulding N, Seal D V
出版信息
Br J Ophthalmol. 1984 May;68(5):316-20. doi: 10.1136/bjo.68.5.316.
Abstract
The specific and non-specific tear proteins have been analysed by means of the ELISA technique to establish the normal and age-related values. There is a linear and related decline of lysozyme and lactoferrin with age, and a similar but unrelated reduction in tear volume. IgA levels gradually decline, while caeruloplasmin and IgG both increase after the fifth decade. The results suggest that tear IgG and caeruloplasmin are probably transudates from the serum, that IgA is secreted independently of tear volume, and that lysozyme and lactoferrin are secreted at the same site but independently of tear volume.
摘要
已通过酶联免疫吸附测定（ELISA）技术分析了特异性和非特异性泪液蛋白，以确定正常和与年龄相关的值。溶菌酶和乳铁蛋白的含量随年龄呈线性相关下降，泪液量也有类似但不相关的减少。免疫球蛋白A（IgA）水平逐渐下降，而血浆铜蓝蛋白和免疫球蛋白G（IgG）在50岁以后均升高。结果表明，泪液IgG和血浆铜蓝蛋白可能是血清的漏出液，IgA的分泌与泪液量无关，溶菌酶和乳铁蛋白在同一部位分泌，但与泪液量无关。
相似文献
1
Normal tear protein profiles and age-related changes.
Br J Ophthalmol. 1984 May;68(5):316-20. doi: 10.1136/bjo.68.5.316.
2
Diagnostic implications of tear protein profiles.
Br J Ophthalmol. 1984 May;68(5):321-4. doi: 10.1136/bjo.68.5.321.
3
Bacteriology and tear protein profiles of the dry eye.
Br J Ophthalmol. 1986 Feb;70(2):122-5. doi: 10.1136/bjo.70.2.122.
4
Human tears: normal protein pattern and individual protein determinations in adults.
Curr Eye Res. 1982;2(5):301-8. doi: 10.3109/02713688209000774.
5
Identification of proteins in small tear volumes with and without size exclusion HPLC fractionation.
Curr Eye Res. 1988 Feb;7(2):163-79. doi: 10.3109/02713688808995746.
6
Proteins in tears from healthy and diseased eyes.
Doc Ophthalmol. 1980 Dec 15;50(1):185-99. doi: 10.1007/BF00161161.
7
Studies of human tear proteins--1. Analysis of tears from normal subjects by crossed immunoelectrophoresis.
Jpn J Ophthalmol. 1982;26(3):314-25.
8
Electrophoresis combined with immunologic identification of human tear proteins.
Invest Ophthalmol Vis Sci. 1989 Aug;30(8):1872-8.
9
[Bactericidal substances in human tear; lysozyme, immunoglobulins and beta-lysin in normal subjects (author's transl)].
Nippon Ganka Gakkai Zasshi. 1979 Sep 10;83(9):1501-8.
10
The effect of ageing and disease on tear constituents.
Trans Ophthalmol Soc U K (1962). 1985;104 ( Pt 4):355-62.
引用本文的文献
1
Impact of COVID-19 on Ocular Surface Health: Infection Mechanisms, Immune Modulation, and Inflammatory Responses.
Viruses. 2025 Jan 6;17(1):68. doi: 10.3390/v17010068.
2
Fluorescence-based reagent and spectrum-based optical reader for lactoferrin detection in tears: differentiating Sjögren's syndrome from non-Sjögren's dry eye syndrome.
Sci Rep. 2024 Jun 24;14(1):14505. doi: 10.1038/s41598-024-65487-2.
3
Sources of Variance in Human Tear Proteomic Samples: Statistical Evaluation, Quality Control, Normalization, and Biological Insight.
Int J Mol Sci. 2024 Jan 26;25(3):1559. doi: 10.3390/ijms25031559.
4
Oxidative Stress in the Protection and Injury of the Lacrimal Gland and the Ocular Surface: are There Perspectives for Therapeutics?
Front Cell Dev Biol. 2022 Mar 11;10:824726. doi: 10.3389/fcell.2022.824726. eCollection 2022.
5
Is dry eye disease the same in young and old patients? A narrative review of the literature.
BMC Ophthalmol. 2022 Feb 22;22(1):85. doi: 10.1186/s12886-022-02269-2.
6
Lactoferrin Ameliorates Dry Eye Disease Potentially through Enhancement of Short-Chain Fatty Acid Production by Gut Microbiota in Mice.
Int J Mol Sci. 2021 Nov 17;22(22):12384. doi: 10.3390/ijms222212384.
7
Age-associated changes in human tear proteome.
Clin Proteomics. 2019 Mar 30;16:11. doi: 10.1186/s12014-019-9233-5. eCollection 2019.
8
Label-Free Detection of Tear Biomarkers Using Hydrogel-Coated Gold Nanoshells in a Localized Surface Plasmon Resonance-Based Biosensor.
ACS Nano. 2018 Sep 25;12(9):9342-9354. doi: 10.1021/acsnano.8b04348. Epub 2018 Sep 14.
9
Protein-directed synthesis of highly monodispersed, spherical gold nanoparticles and their applications in multidimensional sensing.
Sci Rep. 2016 Jun 29;6:28900. doi: 10.1038/srep28900.
10
Corneal Expression of SLURP-1 by Age, Sex, Genetic Strain, and Ocular Surface Health.
Invest Ophthalmol Vis Sci. 2015 Dec;56(13):7888-96. doi: 10.1167/iovs.15-18206.
本文引用的文献
1
CHARACTERISTICS OF AN IMMUNE SYSTEM COMMON TO CERTAIN EXTERNAL SECRETIONS.
J Exp Med. 1965 Jan 1;121(1):101-24. doi: 10.1084/jem.121.1.101.
2
IMMUNOELECTROPHORETIC STUDIES OF THE PROTEIN COMPONENTS OF NORMAL TEARS.
J Immunol. 1964 Oct;93:532-9.
3
The questionably dry eye.
Br J Ophthalmol. 1981 Jan;65(1):2-9. doi: 10.1136/bjo.65.1.2.
4
[Essay about lacrymal proteins during sicca syndromes (author's transl)].
J Fr Ophtalmol. 1980;3(4):263-7.
5
Quantitative tear lysozyme assay: a new technique for transporting specimens.
Br J Ophthalmol. 1980 Sep;64(9):700-4. doi: 10.1136/bjo.64.9.700.
6
Lactoferrin in human ocular tissues.
Am J Ophthalmol. 1980 Jul;90(1):30-7. doi: 10.1016/s0002-9394(14)75074-3.
7
Lactoferrin levels in normal human tears.
Br J Ophthalmol. 1983 Mar;67(3):199-202. doi: 10.1136/bjo.67.3.199.
8
Immunoassay of human tear lysozyme.
Am J Ophthalmol. 1980 Nov;90(5):715-8. doi: 10.1016/s0002-9394(14)75143-8.
9
Further observations on immunoglobulins of external secretions.
Prog Immunobiol Stand. 1970;4:333-5.
10
Lactoferrin, an iron-binding protein in neutrophilic leukocytes.
J Exp Med. 1969 Sep 1;130(3):643-58. doi: 10.1084/jem.130.3.643.
Zotero 插件