泪液蛋白质谱的诊断意义。
Diagnostic implications of tear protein profiles.
作者信息
Mackie I A, Seal D V
出版信息
Br J Ophthalmol. 1984 May;68(5):321-4. doi: 10.1136/bjo.68.5.321.
Abstract
Concentrations of lysozyme, lactoferrin, ceruloplasmin, IgA, and IgG have been measured in tears by the ELISA (enzyme-linked immunosorbent assay) technique. Tears were collected on weighed filter paper discs, after which they were eluted into buffer and transported frozen to a remote laboratory for assay. Patients with sicca, questionably dry eyes and ocular pemphigoid were sampled, as were 54 normal volunteers. Tear protein profiles were established which were unique for each condition and clearly differed from the normal controls. The assay developed is considered suitable for other proteins such as IgE, and could also be used for monitoring the effects of drugs on the lacrimal gland.
摘要
已通过酶联免疫吸附测定（ELISA）技术测量了泪液中溶菌酶、乳铁蛋白、铜蓝蛋白、IgA和IgG的浓度。泪液收集在称重的滤纸圆盘上，之后将其洗脱到缓冲液中，并冷冻运输到远程实验室进行检测。对患有干燥症、疑似干眼症和眼类天疱疮的患者以及54名正常志愿者进行了采样。建立了每种病症特有的泪液蛋白质谱，且与正常对照组明显不同。所开发的检测方法被认为适用于其他蛋白质，如IgE，也可用于监测药物对泪腺的影响。
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