视觉色素初级光化学的共振拉曼研究。视紫质。
Resonance Raman study of the primary photochemistry of visual pigments. Hypsorhodopsin.
作者信息
Pande A J, Callender R H, Ebrey T G, Tsuda M
出版信息
Biophys J. 1984 Mar;45(3):573-6. doi: 10.1016/S0006-3495(84)84194-6.
Abstract
We report here the first resonance Raman results of octopus hypsorhodopsin, a species formed photochemically at very low temperatures from visual pigments. A pump-probe technique was used to obtain Raman spectra from samples at 12 degrees K whose photostationary state mixtures were either hypsorhodopsin rich or hysorhodopsin poor. The data strongly suggest that the Schiff-base linkage between the chromophore of hysorhodopsin and apoprotein is protonated. Further, the results suggest that hypsorhodopsin's chromophore is in some torsionally distorted conformation, possibly having torsional departures from an all-trans isomeric form.
摘要
我们在此报告章鱼视紫红质的首个共振拉曼结果，视紫红质是在极低温度下由视觉色素光化学形成的一种物质。采用泵浦 - 探测技术从处于12K的样品中获取拉曼光谱，这些样品的光稳态混合物要么富含视紫红质，要么视紫红质含量低。数据有力地表明视紫红质的发色团与脱辅基蛋白之间的席夫碱键是质子化的。此外，结果表明视紫红质的发色团处于某种扭转扭曲的构象，可能与全反式异构体形式存在扭转偏差。
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