Schuman R F, Brimfield A A, Hunter K W
Biosci Rep. 1984 Feb;4(2):149-54. doi: 10.1007/BF01120311.
A new assay has been developed for detection of butyrylcholinesterase (EC 3.1.1.8) activity based upon the change in absorbance of phenol red, caused by the release of butyric acid from the substrate. Using commercially available enzyme prepared from horse serum, linear, dose-related decreases in absorbance were obtained, generally with correlation values of 0.965 or greater. The assay was modified and used to detect enzyme activity in the supernatants from primary cultures of mouse hepatocytes. The enzyme-mediated response was inhibited by NN'-diisopropylphosphorodiamidic anhydride, a specific inhibitor of butyrylcholinesterase.
基于底物丁酸释放导致的酚红吸光度变化，已开发出一种用于检测丁酰胆碱酯酶（EC 3.1.1.8）活性的新检测方法。使用从马血清制备的市售酶，获得了吸光度的线性、剂量相关降低，通常相关值为0.965或更高。对该检测方法进行了改进，并用于检测小鼠肝细胞原代培养上清液中的酶活性。酶介导的反应被丁酰胆碱酯酶的特异性抑制剂二异丙基磷酰二胺酸酐抑制。
