van den Hurk R, van Oordt P G
Gen Comp Endocrinol. 1985 Feb;57(2):216-22. doi: 10.1016/0016-6480(85)90266-7.
Rainbow trout fry were treated with equimolar quantities of cortisol, cortisone, androstenedione, and 11 beta-hydroxyandrostenedione, added to the aquarium water during a 4-week period. All four steroids inhibited ovarian growth. Androstenedione did not influence gonadal sex differentiation; the other steroids pushed the sex ratio in the male direction. However, a near to normal sex ratio was observed 300 days after the 11 beta-hydroxyandrostenedione treatment. When added to the food in two different doses during 8 weeks, 11 beta-hydroxyandrostenedione had a pronounced masculinizing effect, and androstenedione did not modify the gonads at all. It is suggested that the two exogenous corticosteroids were probably converted into 11-oxygenated androstenedione derivatives, and that these 11-oxygenated androstenedione derivatives are particularly important in sustaining the differentiation and early development of the testes in rainbow trout. Testosterone does not seem to be indispensable for these processes, because two different doses of testosterone-blocking cyproterone acetate, added to the food for 8 weeks, failed to affect early gonad development.
虹鳟鱼苗在为期4周的时间里，被给予添加到水族箱水中的等摩尔量的皮质醇、可的松、雄烯二酮和11β - 羟基雄烯二酮进行处理。所有这四种类固醇均抑制卵巢生长。雄烯二酮不影响性腺性别分化；其他类固醇则使性别比例朝着雄性方向发展。然而，在11β - 羟基雄烯二酮处理300天后观察到接近正常的性别比例。当在8周内以两种不同剂量添加到食物中时，11β - 羟基雄烯二酮具有显著的雄性化作用，而雄烯二酮对性腺则完全没有影响。有人提出，这两种外源性皮质类固醇可能转化为11 - 氧化雄烯二酮衍生物，并且这些11 - 氧化雄烯二酮衍生物在维持虹鳟鱼睾丸的分化和早期发育中尤为重要。睾酮似乎对于这些过程并非不可或缺，因为添加到食物中8周的两种不同剂量的睾酮阻断剂醋酸环丙孕酮未能影响性腺的早期发育。
