Deeg R, Schmitt U, Rollinger W, Ziegenhorn J
Clin Chem. 1984 May;30(5):790-3.
This new approach to measurement of glycated hemoglobin (Hb A1) is a three-step procedure involving (a) conversion of oxyhemoglobin into NO-hemoglobin by sodium dithionite and sodium nitrite in the presence of inositol hexaphosphate; (b) incubation with haptoglobin, which preferentially binds glycated hemoglobin; and (c) determination of the hemoglobin/haptoglobin complex by its peroxidase activity in acid buffer. The procedure is suitable for automated analysis with instruments that are capable of adding a starting reagent. We describe preliminary adaptations for the Abbott VP and Hitachi 705 analyzers and demonstrate the correlation of results with Hb A1 concentrations determined by ion-exchange chromatography.
这种测量糖化血红蛋白（Hb A1）的新方法是一个三步程序，包括：（a）在肌醇六磷酸存在的情况下，用连二亚硫酸钠和亚硝酸钠将氧合血红蛋白转化为一氧化氮血红蛋白；（b）与触珠蛋白一起孵育，触珠蛋白优先结合糖化血红蛋白；（c）通过其在酸性缓冲液中的过氧化物酶活性测定血红蛋白/触珠蛋白复合物。该程序适用于使用能够添加起始试剂的仪器进行自动分析。我们描述了对雅培VP和日立705分析仪的初步调整，并证明了结果与通过离子交换色谱法测定的Hb A1浓度之间的相关性。
