Ghosh S, Paweletz N
Chromosoma. 1984;89(3):197-200. doi: 10.1007/BF00294999.
Multinucleate cells were induced by colcemid treatment in PtK1 cells in culture. DNA synthesis and mitotic behavior of those cells in which each nucleus contained a single chromosome were studied. More than 80% of such cells showed synchronous DNA synthesis and mitosis in all nuclei. As these genetically different nuclei respond identically to the molecules that initiate DNA synthesis and mitosis, intranuclear control of initiation of DNA synthesis and induction of mitosis by genes on individual chromosomes can be excluded. The occasional occurrence of asynchronous division in multinucleate cells is assumed to result from unequal availability of the inducer molecules to the individual nuclei.
在培养的PtK1细胞中，通过秋水仙酰胺处理诱导产生多核细胞。对每个细胞核含有一条单染色体的细胞的DNA合成和有丝分裂行为进行了研究。超过80%的此类细胞在所有细胞核中表现出同步的DNA合成和有丝分裂。由于这些遗传上不同的细胞核对启动DNA合成和有丝分裂的分子反应相同，因此可以排除单个染色体上的基因对DNA合成起始和有丝分裂诱导的核内控制。多核细胞中偶尔出现的异步分裂被认为是由于诱导分子对各个细胞核的可用性不均等所致。
