Kato H, Sandberg A A
J Cell Biol. 1967 Jul;34(1):35-45. doi: 10.1083/jcb.34.1.35.
Under the influence of Colcemid, a substantial number of binucleate human cells from a line infected with herpes-like virus was found to possess pulverized chromosomes. Although this abnormality was also detected in untreated binucleate cells, the increase in the number of pulverized cells after the addition of Colcemid was too striking to be explained by accumulation of spontaneously occurring cells in response to the mitotic inhibition by Colcemid. Furthermore, the induction of pulverization may be dependent upon Colcemid concentration. These findings imply an involvement of Colcemid in the mechanism of pulverization induction in the system studied. When tritiated thymidine was added to the culture medium simultaneously with Colcemid, the majority of binucleate cells with an intact and a pulverized chromosome set incorporated this isotope into the pulverized set only. This obviously suggests that the nuclei in the binucleate cell are asynchronous in DNA synthesis, and that this asynchrony is intimately related to the induction of the pulverization phenomenon. It seems very probable that the late S phase in the late synthesizing nuclei represents a critical stage at which damage to the chromosomes most readily occurs.
在秋水仙酰胺的影响下，发现来自感染疱疹样病毒的细胞系的大量双核人类细胞具有粉碎的染色体。尽管在未处理的双核细胞中也检测到这种异常，但添加秋水仙酰胺后粉碎细胞数量的增加非常显著，不能用因秋水仙酰胺对有丝分裂的抑制作用而自发产生的细胞积累来解释。此外，粉碎的诱导可能取决于秋水仙酰胺的浓度。这些发现表明秋水仙酰胺参与了所研究系统中粉碎诱导的机制。当将氚标记的胸腺嘧啶核苷与秋水仙酰胺同时添加到培养基中时，大多数具有完整染色体组和粉碎染色体组的双核细胞仅将这种同位素掺入到粉碎的染色体组中。这显然表明双核细胞中的细胞核在DNA合成中是异步的，并且这种异步与粉碎现象的诱导密切相关。很可能晚期合成细胞核中的S期晚期代表了染色体最容易受到损伤的关键阶段。
