细胞松弛素A对兔中性粒细胞趋化性的调节。与其他中性粒细胞功能的比较。

Modulation of rabbit neutrophil chemotaxis by cytochalasin A. A comparison with other neutrophil functions.

作者信息

Elferink J G, Deierkauf M

出版信息

Inflammation. 1984 Mar;8(1):63-72. doi: 10.1007/BF00918354.

Abstract

Chemotaxis and stimulated locomotion by rabbit peritoneal neutrophils are inhibited by cytochalasin A; inhibition is complete with 5 X 10(-7) M cytochalasin A. Under the same conditions exocytosis and activation of the metabolic burst are stimulated maximally with 5 X 10(-7) M cytochalasin A. Inhibition of these function occurs at concentrations higher than 10(-6) M, inhibition being complete with 5 X 10(-6) M cytochalasin A. The sulfhydryl compound glutathione can prevent the inhibitory effect of cytochalasin A on chemotaxis. Inactivation of the sulfhydryl groups on the outer surface of the plasma membrane does not affect the inhibitory effect of cytochalasin A. It is concluded that the cellular target of cytochalasin A has a different significance for chemotaxis as compared with exocytosis and metabolic burst. The possibility that cytochalasin A reacts with sulfhydryl groups, located on a structure closely connected with the inner surface of the plasma membrane, is discussed.

摘要

细胞松弛素A可抑制兔腹膜中性粒细胞的趋化性和刺激运动；5×10⁻⁷ M细胞松弛素A可完全抑制。在相同条件下，5×10⁻⁷ M细胞松弛素A可最大程度地刺激胞吐作用和代谢爆发的激活。高于10⁻⁶ M的浓度会抑制这些功能，5×10⁻⁶ M细胞松弛素A可完全抑制。巯基化合物谷胱甘肽可防止细胞松弛素A对趋化性的抑制作用。质膜外表面巯基的失活不影响细胞松弛素A的抑制作用。得出的结论是，与胞吐作用和代谢爆发相比，细胞松弛素A的细胞靶点对趋化性具有不同的意义。讨论了细胞松弛素A与位于与质膜内表面紧密相连的结构上的巯基发生反应的可能性。