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N-乙酰化氨基糖参与食纤维菌属菌株DR2001与以色列放线菌PK16之间共聚集的证据。

Evidence for the participation of N-acetylated amino sugars in the coaggregation between Cytophaga species strain DR2001 and Actinomyces israelii PK16.

作者信息

Kagermeier A S, London J, Kolenbrander P E

出版信息

Infect Immun. 1984 May;44(2):299-305. doi: 10.1128/iai.44.2.299-305.1984.

DOI:10.1128/iai.44.2.299-305.1984
PMID:6715035
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC263517/
Abstract

Coaggregation between Cytophaga sp. strain DR2001 and Actinomyces israelii PK16 was partially inhibited by certain N-acetylated amino sugars (N-acetylneuraminic acid, N-acetylgalactosamine, and N-acetylglucosamine) and was completely inhibited by the trisaccharide neuraminin-lactose. The monosaccharides exerted their effect at concentrations between 30 to 100 mM, whereas the trisaccharide was an effective inhibitor at significantly lower concentrations. Outer membrane preparations caused A. israelii PK16 cells to aggregate; however, vesicles released from the cell envelope during growth failed to do so. Adherence studies with a non-coaggregating mutant of the cytophaga suggest that the spheroidal hydroxyapatite attachment sites and coaggregation receptors are separate entities.

摘要

噬纤维菌属菌株DR2001与以色列放线菌PK16之间的共聚集作用被某些N - 乙酰化氨基糖（N - 乙酰神经氨酸、N - 乙酰半乳糖胺和N - 乙酰葡糖胺）部分抑制，而被三糖神经氨酸 - 乳糖完全抑制。单糖在30至100 mM的浓度范围内发挥作用，而三糖在显著更低的浓度下就是一种有效的抑制剂。外膜制剂可使以色列放线菌PK16细胞聚集；然而，生长过程中从细胞膜释放的囊泡却不能使其聚集。对噬纤维菌的一个非共聚集突变体进行的黏附研究表明，球状羟基磷灰石附着位点和共聚集受体是不同的实体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da56/263517/294724cb984e/iai00128-0107-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da56/263517/e575d2b39981/iai00128-0106-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da56/263517/294724cb984e/iai00128-0107-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da56/263517/e575d2b39981/iai00128-0106-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/da56/263517/294724cb984e/iai00128-0107-a.jpg

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