Analysis of linkage monosaccharides in human teratocarcinoma cell glycopeptides: paper chromatographic separation of N-acetylglucosaminitol and N-acetylgalactosaminitol.

Simultaneous separation of N-acetylglucosaminitol, N-acetylgalactosaminitol, N-acetylglucosamine and N-acetylgalactosamine from each other was achieved by paper chromatography. The method, which is suitable for analysis of radioactively labeled glycopeptides, allowed identification of N-acetylglucosamine and N-acetylgalactosamine as the linkage sugars in specific glycopeptide fractions isolated from human teratocarcinoma cells of line PA 1.