Marucha P T, Keyes P H, Wittenberger C L, London J
Infect Immun. 1978 Sep;21(3):786-91. doi: 10.1128/iai.21.3.786-791.1978.
Serotype-specific antisera prepared against whole cells of Actinomyces viscosus, A. naeslundii, and A. israeli were labeled with fluorescein dye and used to detect and quantitate antigenically related microorganisms in human dental plaque. By relating the DNA content of the dental plaque microflora to the number of Actinomyces present in the plaque samples, a reproducible method was developed for specifically enumerating five serotypic representatives of this genus found in human plaque.
用荧光染料标记针对黏性放线菌、内氏放线菌和衣氏放线菌全细胞制备的血清型特异性抗血清,并用于检测和定量人类牙菌斑中抗原相关的微生物。通过将牙菌斑微生物群的DNA含量与菌斑样本中存在的放线菌数量相关联,开发了一种可重复的方法,用于特异性计数在人类菌斑中发现的该属的五个血清型代表。