Garrido F, Schirrmacher V, Festenstein H
J Immunogenet. 1977 Feb;4(1):15-27. doi: 10.1111/j.1744-313x.1977.tb00610.x.
Seven mouse tumour cell lines of different aetiology and origin were tested for the expression of surface alloantigens using twenty-four well defined H-2 alloantisera and anti-Thy 1.2. We used a new radioassay that involves antibody-complement treatment of the tumour target cells followed by postlabeling the surviving tumour cells with 14C-thymidine. With a relatively high frequency the anti-H-2 sera were reacting differently with the tumour cells than with respective syngeneic lymphoid cells. Thirty-six anomalous reactions out of 129 investigated were detected. Absorbtion experiments performed with H-2 antigen positive or negative lymphoid cells revealed a striking similarity between these extra-specificities and H-2 specificities of foreign haplotypes. The results are discussed in relation to the biological importance of the extra-specificities, both with regard to origin (derepression) and function (transplantation antigen properties).
使用24种明确的H-2同种异体抗血清和抗Thy 1.2，对7种不同病因和来源的小鼠肿瘤细胞系进行表面同种异体抗原表达检测。我们采用了一种新的放射测定法，该方法包括对肿瘤靶细胞进行抗体-补体处理，然后用14C-胸腺嘧啶核苷对存活的肿瘤细胞进行后标记。抗H-2血清与肿瘤细胞的反应频率相对较高，与相应的同基因淋巴细胞不同。在129次研究中检测到36次异常反应。用H-2抗原阳性或阴性淋巴细胞进行的吸收实验表明，这些额外特异性与外来单倍型的H-2特异性之间存在惊人的相似性。本文从额外特异性的生物学重要性出发，从起源（去抑制）和功能（移植抗原特性）两方面对结果进行了讨论。
