Fukuda M, Dell A, Fukuda M N
J Biol Chem. 1984 Apr 25;259(8):4782-91.
The structure of lactosaminoglycan prepared from Band 3 glycoprotein of umbilical cord blood erythrocytes was elucidated. The glycopeptides were digested by endo-beta-galactosidase and oligosaccharides, core glycopeptides, and intact glycopeptides were analyzed by permethylation, exoglycosidase digestion, and fast atom bombardment mass spectrometry. The structure of one of the major components was found to be: sequence in text This structure is unique in that 1) two linear polylactosaminyl chains are attached to the core portion, 2) the polylactosaminyl side chain is much longer on the Man alpha 1----6 side than on the Man alpha 1----3 side, 3) alpha 2----3-linked sialic acid is present at the terminal of the long polylactosaminyl side chain, whereas alpha 2----6-linked sialic acid is present in the other short side chain, and 4) fucose is linked only to the external position of the lactosaminyl side chain and fucose, alpha 1----6-linked to the innermost N-acetylglucosamine, is absent.
阐明了从脐带血红细胞带3糖蛋白制备的乳糖胺聚糖的结构。糖肽用内切β-半乳糖苷酶消化，寡糖、核心糖肽和完整糖肽通过全甲基化、外切糖苷酶消化和快原子轰击质谱分析。发现其中一种主要成分的结构为：文本中的序列 这种结构的独特之处在于：1）两条线性聚乳糖胺链连接到核心部分；2）聚乳糖胺侧链在Manα1----6侧比在Manα1----3侧长得多；3）α2----3连接的唾液酸存在于长聚乳糖胺侧链的末端，而α2----6连接的唾液酸存在于另一条短侧链中；4）岩藻糖仅连接到乳糖胺侧链的外部位置，且不存在与最内层N-乙酰葡糖胺α1----6连接的岩藻糖。
