Spooncer E, Fukuda M, Klock J C, Oates J E, Dell A
J Biol Chem. 1984 Apr 25;259(8):4792-801.
Lactosaminoglycan was isolated from human granulocytes and the structure of neutral lactosaminoglycan was elucidated. The lactosaminoglycan glycopeptides and lactosaminoglycan saccharides obtained by hydrazinolysis were analyzed by permethylation. In addition, the lactosaminoglycan was digested by endo-beta-galactosidase and the "core" portion and released oligosaccharides were analyzed by specific glycosidases, permethylation, and fast atom bombardment mass spectrometry. The structure of the major component in the neutral lactosaminoglycan was found to be: sequence in text where m + n + o + p greater than 6, and the mean value of fucose content = 4.5. This structure is unique in that 1) four linear polylactosaminyl chains are attached to the core portions, 2) N-acetylglucosamine residues in the polylactosaminyl side chains are substituted with fucose through an alpha 1----3 linkage and at least 1 mol of Gal beta 1----4(Fuc alpha 1----3)GlcNAc terminal structure is present, and 3) the tetraantennary core is a major component. Since this polyfucosylated lactosaminoglycan is abundantly present in human granulocytes, we propose that this lactosaminoglycan is a major carrier for the granulocyte-specific antigen, Gal beta 1----4(Fuc alpha 1----3)GlcNAc, which is recognized by the My-1 monoclonal antibody (Huang, L. C., Civin, C. I., Magnani, J. L., Shaper, J. H., and Ginsburg, V. (1983) Blood 61, 1020-1023).
从人粒细胞中分离出乳糖氨基聚糖，并阐明了中性乳糖氨基聚糖的结构。通过全甲基化分析了经肼解得到的乳糖氨基聚糖糖肽和乳糖氨基聚糖糖类。此外，用内切β-半乳糖苷酶消化乳糖氨基聚糖，并用特异性糖苷酶、全甲基化和快原子轰击质谱分析“核心”部分和释放的寡糖。发现中性乳糖氨基聚糖中主要成分的结构为：文中序列，其中m + n + o + p大于6，岩藻糖含量的平均值 = 4.5。该结构的独特之处在于：1）四条线性多乳糖胺链连接到核心部分；2）多乳糖胺侧链中的N-乙酰葡糖胺残基通过α1----3连接被岩藻糖取代，并且至少存在1摩尔的Galβ1----4（Fucα1----3）GlcNAc末端结构；3）四天线核心是主要成分。由于这种多岩藻糖基化的乳糖氨基聚糖大量存在于人粒细胞中，我们提出这种乳糖氨基聚糖是粒细胞特异性抗原Galβ1----4（Fucα1----3）GlcNAc的主要载体，该抗原可被My-1单克隆抗体识别（黄，L.C.，西文，C.I.，马尼亚尼，J.L.，沙珀，J.H.，和金斯伯格，V.（1983年）《血液》61，1020 - 1023）。
